| http://purl.uniprot.org/citations/17416588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17416588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17416588 | http://www.w3.org/2000/01/rdf-schema#comment | "Intestinal epithelial cells (IEC) play an immunoregulatory role in the intestine. This role involves cell-cell interactions with intraepithelial lymphocytes that may also play a role in some enteropathies. The discovery of the RGD motif-containing Protein ADAM-15 (a disintegrin and metalloprotease-15) raises the question of its involvement in these cell-cell interactions. Cell adhesion assays were performed using the Jurkat E6.1 T cell line as a model of T lymphocytes and Caco2-BBE monolayers as a model of intestinal epithelia. Our results show that an anti-ADAM-15 ectodomain antibody inhibited the attachment of Jurkat cells on Caco2-BBE monolayers. Overexpression of ADAM-15 in Caco2-BBE cells enhanced Jurkat cell binding, and overexpression of ADAM-15 in Jurkat cells enhanced their aggregation. Mutagenesis experiments showed that both the mutation of ADAM-15 RGD domain or the deletion of its cytoplasmic tail decreased these cell-cell interactions. Moreover, wound-healing experiments showed that epithelial ADAM-15-mediated Jurkat cell adhesion to Caco2-BBE cells enhances the mechanisms of wound repair. We also found that ADAM-15-mediated aggregation of Jurkat cells increases the expression of tumor necrosis factor-alpha mRNA. These results demonstrate the following: 1) ADAM-15 is involved in heterotypic adhesion of intraepithelial lymphocytes to IEC as well as in homotypic aggregation of T cells; 2) both the RGD motif and the cytoplasmic tail of ADAM-15 are involved for these cell-cell interactions; and 3) ADAM-15-mediated cell-cell interactions are involved in mechanisms of epithelial restitution and production of pro-inflammatory mediators. Altogether these findings point to ADAM-15 as a possible therapeutic target for prevention of inappropriate T cell activation involved in some pathologies."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m700158200"xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m700158200"xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Nguyen H.T."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Nguyen H.T."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Yan Y."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Yan Y."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Charrier L."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Charrier L."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Dalmasso G."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Dalmasso G."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Merlin D."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Merlin D."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Sitaraman S.V."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Sitaraman S.V."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Gewirtz A.T."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Gewirtz A.T."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Laboisse C.L."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/author | "Laboisse C.L."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17416588 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |