| http://purl.uniprot.org/citations/17456551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17456551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17456551 | http://www.w3.org/2000/01/rdf-schema#comment | "Beta-arrestins are known to act as endocytic adaptors by recruiting the clathrin adaptor protein 2 (AP-2) complex to G-protein-coupled receptors (GPCRs), linking them to clathrin-coated pits (CCPs) for internalization. They also act as signaling molecules connecting GPCRs to different downstream effectors. We have previously shown that stimulation of the angiotensin II (Ang II) type 1 receptor (AGTR1, hereafter referred to as AT1R), a member of the GPCR family, promotes the formation of a complex between beta-arrestin, the kinase Src and AP-2. Here, we report that formation of such a complex is involved in the AT1R-mediated tyrosine phosphorylation of beta2-adaptin, the subunit of AP-2 involved in binding beta-arrestin. We identify a crucial tyrosine residue in the ear domain of beta2-adaptin and show in vitro that the phosphorylation of this site regulates the interaction between beta-arrestin and beta2-adaptin. Using fluorescently tagged proteins combined with resonance energy transfer and image cross-correlation spectroscopy approaches, we show in live cells that beta2-adaptin phosphorylation is an important regulatory process for the dissociation of beta-arrestin-AP-2 complexes in CCPs. Finally, we show that beta2-adaptin phosphorylation is involved in the early steps of receptor internalization. Our findings not only unveil beta2-adaptin as a new Src target during AT1R internalization, but also support the role of receptor-mediated signaling in the control of clathrin-dependent endocytosis of receptors."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.03444"xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.03444"xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Hamdan F.F."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Hamdan F.F."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Bouvier M."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Bouvier M."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Zimmerman B."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Zimmerman B."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Comeau J."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Comeau J."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Fessart D."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Fessart D."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Laporte S.A."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Laporte S.A."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Simaan M."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Simaan M."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Wiseman P.W."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/author | "Wiseman P.W."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/17456551 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |