| http://purl.uniprot.org/citations/17504815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17504815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17504815 | http://www.w3.org/2000/01/rdf-schema#comment | "The microtubule-associated protein AtMAP65-1 from Arabidopsis thaliana dimerizes and forms 25 nm cross-bridges between microtubules, but the exact mechanism is unknown. Here, we used the predicted three-dimensional structure of AtMAP65-1 as a basis for analyzing the actual cross-bridging in detail. Fold-recognition predicts that AtMAP65-1 contains four coiled-coil domains and a flexible extended loop. The length of these coiled-coil domains is about 25 nm, suggesting that one molecule could span the gap, hence forming an antiparallel overlapping dimer instead of an end-to-end dimer. We then tested this model by using truncations of AtMAP65-1. EDC {[3-(dimethylamino) propyl] carbodiimide} cross-linking analysis indicated that the N-terminus of the rod domain of AtMAP65-1 (amino acids 1-339) binds to the C-terminus of the rod domain (amino acids 340-494) and also participates in connecting the two antiparallel proteins in the cross-bridge. Nevertheless, microtubules can still form bundles in the presence of AtMAP65-1 340-587 (amino acids 340-587) or AtMAP65-1 1-494 (amino acids 1-494). Comparing the cold stability of microtubule bundles induced by full-length AtMAP65-1 with that of AtMAP65-1 340-587 or AtMAP65-1 1-494, we conclude that AtMAP65-1 495-587 acts as a flexible extended loop, playing a crucial role in binding to and stabilizing microtubules in the AtMAP65-1 cross-bridge."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcm059"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcm059"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Yuan M."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Yuan M."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Mao T."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/author | "Mao T."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/pages | "866-874"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/pages | "866-874"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/title | "The AtMAP65-1 cross-bridge between microtubules is formed by one dimer."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/title | "The AtMAP65-1 cross-bridge between microtubules is formed by one dimer."xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/17504815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17504815 |
| http://purl.uniprot.org/citations/17504815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17504815 |