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http://purl.uniprot.org/citations/18067895http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18067895http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18067895http://www.w3.org/2000/01/rdf-schema#comment"

Background

We determined previously that hypoxia results in increased 15-lipoxygenase type 2 (15-LOX-2) expression and CXCL8 secretion in macrophages. This study sought to determine whether 15-LOX-2 expression links directly with the secretion of inflammatory molecules in macrophages and also investigated its subsequent effects on T cell migration.

Methods

Adenovirus-mediated gene delivery caused overexpression of 15-LOX-2 in human macrophages. We used cytometric bead array to measure chemokine secretion, and assessed T cell migration by counting cells in chemotaxis chambers. Expression of chemokine receptors was determined by FACS analysis. Using siRNA, we reduced 15-LOX-2 expression in human macrophages. We used scrambled siRNA as control.

Results

Macrophages that overexpress 15-LOX-2 showed increased secretion of chemokine CXCL10 after 24h incubation. In addition, preconditioned medium from 15-LOX-2-overexpressing cells increased T cell migration and surface expression of CXCR3, the CXCL10 receptor. Knockdown of 15-LOX-2 expression decreased CXCL10 secretion from hypoxic macrophages and also reduced T cell migration.

Conclusion

In macrophages, overexpression of 15-LOX-2 results in increased secretion of CXCL10 and CCL2. Products released in response to increased 15-LOX-2 activation lead to increased expression of CD69, the T cell activation marker as well as increased T cell migration. Therefore, increased expression of 15-LOX-2 induced by hypoxia may participate in T cell recruitment in diseases such as atherosclerosis."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.org/dc/terms/identifier"doi:10.1016/j.atherosclerosis.2007.10.027"xsd:string
http://purl.uniprot.org/citations/18067895http://purl.org/dc/terms/identifier"doi:10.1016/j.atherosclerosis.2007.10.027"xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Wiklund O."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Wiklund O."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Boren J."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Boren J."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Akyuerek L.M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Akyuerek L.M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Danielsson K.N."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Danielsson K.N."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Forsberg G.B."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Forsberg G.B."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Hulten L.M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Hulten L.M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Ingelsten M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Ingelsten M."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Jirholt P."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Jirholt P."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Rydberg E.K."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Rydberg E.K."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Ullstroem C."xsd:string
http://purl.uniprot.org/citations/18067895http://purl.uniprot.org/core/author"Ullstroem C."xsd:string
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