| http://purl.uniprot.org/citations/18072184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18072184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18072184 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA segregation in mammalian cells during mitosis is an essential cellular process that is mediated by a specific subchromosomal protein complex, the kinetochore. Malfunction of this complex results in aneuploidy and can cause cancer. A subkinetochore complex, the "inner kinetochore", is present at the centromere during the entire cell cycle. Its location seems to be defined by the settlement of CENP-A (CENH3), which replaces histone H3 in centromeric nucleosomes. This suggests that CENP-A can recruit further inner kinetochore proteins by direct binding. Surprisingly, intense in vitro studies could not identify an interaction of CENP-A with any other inner kinetochore protein. Instead, centromere identity seems to be maintained by a unique nucleosome, which might have a modified structure or epigenetic state that serves to distinguish the centromere from the rest of the chromosome. We investigated the association of CENP-A and CENP-B by fluorescence intensity and lifetime-based FRET measurements in living human HEp-2 cells. We observed Förster resonance energy transfer (FRET) between CENP-A and CENP-B at centromere locations; this indicates that these proteins are in the molecular vicinity (<10 nm) of each other. In addition, we analysed protein-protein interactions within the centromeric nucleosome. We could detect energy transfer between CENP-A and histone H4 as well as between CENP-A molecules themselves. On the other hand, no FRET was detected between CENP-A and H2A.1 or H3.1. Our data support the view that two CENP-A molecules are packed with H4, but not with H3, in a single centromeric nucleosome."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.200700358"xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.200700358"xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Hoffmann B."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Hoffmann B."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Hoischen C."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Hoischen C."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Diekmann S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Diekmann S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Benndorf K."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Benndorf K."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Biskup C."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Biskup C."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Orthaus S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Orthaus S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Ohndorf S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/author | "Ohndorf S."xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/pages | "77-92"xsd:string |
| http://purl.uniprot.org/citations/18072184 | http://purl.uniprot.org/core/pages | "77-92"xsd:string |