| http://purl.uniprot.org/citations/18256029 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18256029 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18256029 | http://www.w3.org/2000/01/rdf-schema#comment | "ALG-2, a prototypic member of the penta-EF-hand protein family, interacts with Alix at its C-terminal Pro-rich region containing four tandem PXY repeats. Human phospholipid scramblase 3 (PLSCR3) has a similar sequence (ABS-1) in its N-terminal region. In the present study, we found that ALG-2 interacts with PLSCR3 expressed in HEK293 cells in a Ca(2+)-dependent manner by co-immunoprecipitation, pulldown with glutathione S-transferase (GST) fused ALG-2 and an overlay assay using biotin-labeled ALG-2. The GST fusion protein of an alternatively spliced isoform of ALG-2, GST-ALG-2(DeltaGF122), pulled down green fluorescent protein (GFP)-fused PLSCR3 but not GFP Alix. Deletion of a region containing ABS-1 was not sufficient to abrogate the binding. A second ALG-2-binding site (ABS-2) was essential for interaction with ALG-2(DeltaGF122). Real-time interaction analyses with a surface plasmon resonance biosensor using synthetic oligopeptides and recombinant proteins corroborated direct Ca(2+)-dependent binding of ABS-1 to ALG-2 and that of ABS-2 to ALG-2 as well as to ALG-2(DeltaGF122). The sequence of ABS-2 contains multiple prolines and two phenylalanines, among which Phe(49) was found to be critical, because its substitution with Ala or Tyr caused a loss of binding ability by pulldown assays using oligopeptide-immobilized beads. ALG-2-interacting proteins were classified into two groups based on binding ability to ALG-2(DeltaGF122): (i) isoform-non-interactive (ABS-1) types, including Alix, annexin A7, annexin A11, and TSG101 and (ii) isoform-interactive (ABS-2) types including PLSCR3, PLSCR4 and Sec31A. GST-pulldown assays using single amino acid-substituted ALG-2 mutants revealed differences in binding specificities between the two groups, suggesting structural flexibility in ALG-2-ligand complex formation."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m800717200"xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m800717200"xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Mizuno T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Mizuno T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Yoshida H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Yoshida H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Maki M."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Shibata H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Shibata H."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Inuzuka T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Inuzuka T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Kakiuchi T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/author | "Kakiuchi T."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/pages | "9623-9632"xsd:string |
| http://purl.uniprot.org/citations/18256029 | http://purl.uniprot.org/core/pages | "9623-9632"xsd:string |