| http://purl.uniprot.org/citations/1834645 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1834645 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1834645 | http://www.w3.org/2000/01/rdf-schema#comment | "We report the characterization of two functional domains of the metastasis-associated 67-kDa laminin receptor (67-LR). Using synthetic peptides deduced from the cDNA sequence of the 37-kDa precursor of the laminin receptor (37-LRP) as well as their corresponding affinity-purified polyclonal antibodies, we identified a unique laminin binding site as well as a membrane-associated domain of the receptor. In laminin dot blot and solid phase radioligand assays, a 20 amino acid synthetic peptide (IPCNNKGAHSVGLMWWMLAR, amino acid residues 161-180, designated peptide G) specifically bound to laminin with high affinity (Kd = 5 x 10(-8) M). Peptide G also specifically eluted the 67-LR from a laminin affinity column. Peptide G and laminin reacted with a 1:1 stoichiometry, suggesting that there is one recognition site on laminin for the peptide G domain. Immunofluorescence studies, performed on permeabilized and nonpermeabilized human A2058 melanoma cells using 10 different affinity-purified antibodies to distinct regions of the 37-LRP, identified an unusually short membrane-associated domain that was consistent with a computer predicted transmembrane domain (residues 86-101). Our data demonstrate for the first time that the 37-LRP has two functional domains consistent with the characteristics of the mature 67-LR. Furthermore, we propose peptide G as a potential inhibitor of tumor cell interactions with laminin."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)54943-7"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)54943-7"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Castronovo V."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Castronovo V."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Sobel M.E."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Sobel M.E."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Taraboletti G."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/author | "Taraboletti G."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/pages | "20440-20446"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/pages | "20440-20446"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/title | "Functional domains of the 67-kDa laminin receptor precursor."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/title | "Functional domains of the 67-kDa laminin receptor precursor."xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/volume | "266"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://purl.uniprot.org/core/volume | "266"xsd:string |
| http://purl.uniprot.org/citations/1834645 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1834645 |
| http://purl.uniprot.org/citations/1834645 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1834645 |
| http://purl.uniprot.org/citations/1834645 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1834645 |
| http://purl.uniprot.org/citations/1834645 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1834645 |