| http://purl.uniprot.org/citations/18354232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18354232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18354232 | http://www.w3.org/2000/01/rdf-schema#comment | "LPS-binding protein (LBP) is a central mediator that transfers LPS to CD14 to initiate TLR4-mediated proinflammatory response. However, a possibility of another LPS transfer molecule has been suggested because LBP-deficient mice showed almost normal inflammatory response after LPS injection. In this study, we describe the novel finding that high mobility group box 1 protein (HMGB1) recently identified as a mediator of sepsis has a function of LPS transfer for a proinflammatory response. We used ELISA and surface plasmon resonance to show that HMGB1 binds LPS in a concentration-dependent manner and that the binding is stronger to lipid A moiety than to the polysaccharide moiety of LPS. This binding was inhibited by LBP and polymyxin B. Using native PAGE and fluorescence-based LPS transfer analyses, we show that HMGB1 can catalytically disaggregate and transfer LPS to both soluble CD14 protein and to human PBMCs in a dose-dependent manner. However, this effect was dramatically reduced to the baseline level when HMGB1 was heat inactivated. Furthermore, a mixture of HMGB1 and LPS treatment results in a higher increase in TNF-alpha production in human PBMCs and peripheral blood monocytes than LPS or HMGB1 treatment alone or their summation. Thus, we propose that HMGB1 plays an important role in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.180.7.5067"xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.180.7.5067"xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Shin J.S."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Shin J.S."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Kim E.S."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Kim E.S."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Choi J.E."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Choi J.E."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Oh Y.J."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Oh Y.J."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Youn J.H."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/author | "Youn J.H."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/pages | "5067-5074"xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/pages | "5067-5074"xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/title | "High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/title | "High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes."xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/volume | "180"xsd:string |
| http://purl.uniprot.org/citations/18354232 | http://purl.uniprot.org/core/volume | "180"xsd:string |