| http://purl.uniprot.org/citations/18386027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18386027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18386027 | http://www.w3.org/2000/01/rdf-schema#comment | "Cutaneous tissue injury, both in vivo and in vitro, initiates activation of a "wound repair" transcriptional program. One such highly induced gene encodes plasminogen activator inhibitor type-1 (PAI-1, SERPINE1). PAI-1-GFP, expressed as a fusion protein under inducible control of +800 bp of the wound-activated PAI-1 promoter, prominently "marked" keratinocyte migration trails during the real-time of monolayer scrape-injury repair. Addition of active recombinant PAI-1 to wounded wild-type keratinocyte monolayers as well as to PAI-1(-/-) MEFs and PAI-1(-/-) keratinocytes significantly stimulated directional motility above basal levels in all cell types. PAI-1 expression knockdown or antibody-mediated functional inhibition, in contrast, effectively attenuated injury repair. The defect in wound-associated migratory activity as a consequence of antisense-mediated PAI-1 down-regulation was effectively reversed by addition of recombinant PAI-1 immediately after scrape injury. One possible mechanism underlying the PAI-1-dependent motile response may involve fine control of the keratinocyte substrate detachment/re-attachment process. Exogenous PAI-1 significantly enhanced keratinocyte spread cell "footprint" area while PAI-1 neutralizing antibodies, but not control non-immune IgG, effectively inhibited spreading with apoptotic hallmarks evident within 24 h. Importantly, PAI-1 not only stimulated keratinocyte adhesion and wound-initiated planar migration but also rescued keratinocytes from plasminogen-induced substrate detachment/anoikis. The early transcriptional response of the PAI-1 gene to monolayer trauma and its prominence in the injury repair genetic signature are consistent with its function as both a survival factor and regulator of the time course of epithelial migration as part of the cutaneous injury response program."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00403-008-0845-2"xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00403-008-0845-2"xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins C.E."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins C.E."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins P.J."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins P.J."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Battista A."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Battista A."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins S.P."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Higgins S.P."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Mullen A."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Mullen A."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Providence K.M."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Providence K.M."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Samarakoon R."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Samarakoon R."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Wilkins-Port C.E."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/author | "Wilkins-Port C.E."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/name | "Arch. Dermatol. Res."xsd:string |
| http://purl.uniprot.org/citations/18386027 | http://purl.uniprot.org/core/name | "Arch. Dermatol. Res."xsd:string |