| http://purl.uniprot.org/citations/18515354 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18515354 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18515354 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/18515354 | http://www.w3.org/2000/01/rdf-schema#comment | "The hypothetical protein C7orf24 has been implicated as a cancer marker with a potential role in cell proliferation. We have identified C7orf24 as gamma-glutamyl cyclotransferase (GGCT) that catalyzes the formation of 5-oxoproline (pyroglutamic acid) from gamma-glutamyl dipeptides and potentially plays a significant role in glutathione homeostasis. In the present study we have identified the first cDNA clones encoding a gamma-glutamyl cyclotransferase. The GGCT gene is located on chromosome 7p14-15 and consists of four exons that span 8 kb. The primary sequence is 188 amino acids in length and is unlike any protein of known function. We crystallized functional recombinant gamma-glutamyl cyclotransferase and determined its structure at 1.7 A resolution. The enzyme is a dimer of 20,994-Da subunits. The topology of GGCT is unrelated to other enzymes associated with cyclotransferase-like activity. The fold was originally classified as "BtrG-like," a small family that only includes structures of hypothetical proteins from Mus musculus, Escherichia coli, Pyrococcus horikoshii, and Arabidopsis thaliana. Since this is the first member of this family with a defined function, we propose to refer to this structure as the gamma-glutamyl cyclotransferase fold. We have identified a potential active site pocket that contains a highly conserved glutamic acid (Glu(98)) and propose that it acts as a general acid/base in the reaction mechanism. Mutation of Glu(98) to Ala or Gln completely inactivates the enzyme without altering the overall fold."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m803623200"xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m803623200"xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Clark A.G."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Clark A.G."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Liu D."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Board P.G."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Board P.G."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Oakley A.J."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Oakley A.J."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Coggan M."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/author | "Coggan M."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/pages | "22031-22042"xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/pages | "22031-22042"xsd:string |
| http://purl.uniprot.org/citations/18515354 | http://purl.uniprot.org/core/title | "The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase: an essential enzyme in the gamma-glutamyl cycle."xsd:string |