| http://purl.uniprot.org/citations/18591676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18591676 | http://www.w3.org/2000/01/rdf-schema#comment | "Actin-binding proteins (ABPs) regulate the assembly of actin filaments (F-actin) into networks and bundles that provide the structural integrity of the cell. Two of these ABPs, filamin and alpha-actinin, have been extensively used to model the mechanical properties of actin networks grown in vitro; however, there is a lack in the understanding of how the molecular interactions between ABPs and F-actin regulate the dynamic properties of the cytoskeleton. Here, we present a native-like assay geometry to test the rupture force of a complex formed by an ABP linking two quasiparallel actin filaments. We readily demonstrate the adaptability of this assay by testing it with two different ABPs: filamin and alpha-actinin. For filamin/actin and alpha-actinin/actin, we measured similar rupture forces of 40-80 pN for loading rates between 4 and 50 pN/s. Both ABP unfolding and conformational transition events were observed, demonstrating that both are important and may be a significant mechanism for the temporal regulation of the mechanical properties of the actin cytoskeleton. With this modular, single-molecule assay, a wide range of ABP/actin interactions can be studied to better understand cytoskeletal and cell dynamics."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0706124105"xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Lee H."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Nakamura F."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Lang M.J."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Kamm R.D."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Ferrer J.M."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/author | "Pelz B."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/pages | "9221-9226"xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/title | "Measuring molecular rupture forces between single actin filaments and actin-binding proteins."xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://purl.uniprot.org/core/volume | "105"xsd:string |
| http://purl.uniprot.org/citations/18591676 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18591676 |
| http://purl.uniprot.org/citations/18591676 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18591676 |
| http://purl.uniprot.org/uniprot/P68135#attribution-C6FDF9E0E756C7EC6DD231D50A0289EC | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18591676 |