| http://purl.uniprot.org/citations/18723511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18723511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18723511 | http://www.w3.org/2000/01/rdf-schema#comment | "Members of the Nedd4 family of E3 ubiquitin ligases bind the L domain in avian sarcoma virus (ASV) Gag and facilitate viral particle release. Translational fusion of ASV Gag with an L domain deletion (Deltap2b) to proteins that comprise ESCRT-I, -II, and -III (the endocytic sorting complexes required for transport) rescued both Gag ubiquitination and particle release from cells. The ESCRT-I factors Vps37C or Tsg101 were more effective in rescue of Gag/Deltap2b budding than the ESCRT-II factor Eap20 or the ESCRT-III component CHMP6. Thus ESCRT components can substitute for Nedd4 family members in ASV Gag release. Unlike wild type, ASV Gag/Deltap2b -ESCRT chimeras failed to co-immunoprecipitate with co-expressed hemagglutinin-tagged Nedd4, indicating that Nedd4 was not stably associated with these Gag fusions. Release of the Gag-ESCRT-I or -II fusions was inhibited by a dominant negative mutant of Vps4 ATPase similar to wild type ASV Gag. In contrast to ASV Gag, HIV-1 Gag containing an L domain inactivating mutation (P7L) was efficiently rescued by fusion to a component of ESCRT-III (Chmp6) but not ESCRT-II (Eap20). Depletion of the endogenous pool of Eap20 (ESCRT-II) had little effect on HIV-1 Gag release but blocked ASV Gag release. In contrast, depletion of the endogenous pool of Vps37C (ESCRT-I) had little effect on ASV but blocked HIV-1 Gag release. Furthermore, an N-terminal fragment of Chmp6 inhibited both HIV-1 and ASV Gag release in a dominant negative manner. Taken together, these results indicate that ASV and HIV-1 Gag utilize different combinations of ESCRT proteins to facilitate the budding process, although they share some common elements."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m804157200"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m804157200"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Carter C."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Carter C."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Leis J."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Leis J."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Medina G."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Medina G."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Pincetic A."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/author | "Pincetic A."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/pages | "29822-29830"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/pages | "29822-29830"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/title | "Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/title | "Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process."xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18723511 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18723511 |
| http://purl.uniprot.org/citations/18723511 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18723511 |