| http://purl.uniprot.org/citations/18771725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18771725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18771725 | http://www.w3.org/2000/01/rdf-schema#comment | "AS160 (AKT substrate of 160 kDa) is an important mediator of GLUT4 (glucose transporter 4) translocation and glucose-uptake in adipocytes and muscle cells. In our study we have identified a novel splice variant of AS160 (variant 2 of AS160, AS160_v2) that lacks exon 11 and 12. The protein is phosphorylated in response to insulin via the PI3K/AKT pathway. Expression of this splice variant in human tissues from different donors was examined with quantitative RT-PCR. Our data reveal a tissue specific distribution pattern of both isoforms with highest overall expression of AS160_v2. To investigate the function of the novel splice variant we established the doxycycline-inducible expression of the protein in a rat myoblast cell line co-expressing GLUT4-myc. In contrast to data reported for the full-length AS160 protein, over expression and activation of transcript variant 2 in this cell line increased GLUT4 translocation and glucose-uptake rates in response to insulin and IGF-1 but not in response to AICAR or metformin. Immunofluorescence based studies indicated a direct association of AS160_v2 with GLUT4 under basal but not under insulin-stimulated conditions. Additionally, over expression of AS160_v2 slightly improved glucose-uptake rates in a model of insulin resistance but was not able to fully prevent induction of insulin resistance. This was accompanied with decreased phosphorylation of AS160_v2 and AKT. Taken together, our data suggest a tissue specific distribution of full-length AS160 and the novel AS160 splice variant (AS160_v2) indicating different functions. In contrast to full-length AS160, transcript variant 2 of AS160 seems to be a novel regulator of glucose transport that positively influences glucose-uptake rates."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cellsig.2008.08.010"xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cellsig.2008.08.010"xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Gassenhuber J."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Gassenhuber J."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Dittrich W."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Dittrich W."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Tennagels N."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Tennagels N."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Baus D."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Baus D."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "De Hoop M."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "De Hoop M."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Heermeier K."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Heermeier K."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Metz-Weidmann C."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Metz-Weidmann C."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Welte S."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/author | "Welte S."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/name | "Cell. Signal."xsd:string |
| http://purl.uniprot.org/citations/18771725 | http://purl.uniprot.org/core/name | "Cell. Signal."xsd:string |