| http://purl.uniprot.org/citations/18801371 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18801371 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18801371 | http://www.w3.org/2000/01/rdf-schema#comment | "Mutation of the Caenorhabditis elegans gene unc-89 results in disorganization of muscle A-bands. unc-89 encodes a giant polypeptide (900 kDa) containing a DH domain followed by a PH domain at its N terminus, which is characteristic of guanine nucleotide exchange factor proteins for Rho GTPases. To obtain evidence that the DH-PH region has activity toward specific Rho family small GTPases, we conducted an experiment using the yeast three-hybrid system. The DH-PH region of UNC-89 has exchange activity for RHO-1 (C. elegans RhoA), but not for CED-10 (C. elegans Rac), MIG-2 (C. elegans RhoG), or CDC-42 (C. elegans Cdc42). The DH domain alone has similar activity for RHO-1. An in vitro binding assay demonstrates interaction between the DH-PH region of UNC-89 and each of the C. elegans Rho GTPases. Partial knockdown of rho-1 in C. elegans adults showed a pattern of disorganization of myosin thick filaments similar to the phenotype caused by unc-89 (su75), a mutant allele in which all of the isoforms containing the DH-PH region are missing. Taken together, we propose a model in which the DH-PH region of UNC-89 activates RHO-1 GTPase for organization of myosin filaments in C. elegans muscle cells."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.08.083"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.08.083"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Xiong G."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Xiong G."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Blangy A."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/author | "Blangy A."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/pages | "747-752"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/pages | "747-752"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/title | "The DH-PH region of the giant protein UNC-89 activates RHO-1 GTPase in Caenorhabditis elegans body wall muscle."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/title | "The DH-PH region of the giant protein UNC-89 activates RHO-1 GTPase in Caenorhabditis elegans body wall muscle."xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/volume | "383"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://purl.uniprot.org/core/volume | "383"xsd:string |
| http://purl.uniprot.org/citations/18801371 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18801371 |
| http://purl.uniprot.org/citations/18801371 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18801371 |