| http://purl.uniprot.org/citations/19056867 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19056867 | http://www.w3.org/2000/01/rdf-schema#comment | "Normal human urine contains large numbers of exosomes, which are 40-to 100-nm vesicles that originate as the internal vesicles in multivesicular bodies from every renal epithelial cell type facing the urinary space. Here, we used LC-MS/MS to profile the proteome of human urinary exosomes. Overall, the analysis identified 1132 proteins unambiguously, including 177 that are represented on the Online Mendelian Inheritance in Man database of disease-related genes, suggesting that exosome analysis is a potential approach to discover urinary biomarkers. We extended the proteomic analysis to phosphoproteomic profiling using neutral loss scanning, and this yielded multiple novel phosphorylation sites, including serine-811 in the thiazide-sensitive Na-Cl co-transporter, NCC. To demonstrate the potential use of exosome analysis to identify a genetic renal disease, we carried out immunoblotting of exosomes from urine samples of patients with a clinical diagnosis of Bartter syndrome type I, showing an absence of the sodium-potassium-chloride co-transporter 2, NKCC2. The proteomic data are publicly accessible at http://dir.nhlbi.nih.gov/papers/lkem/exosome/."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.org/dc/terms/identifier | "doi:10.1681/asn.2008040406"xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Hoffert J.D."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Knepper M.A."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Pisitkun T."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Kleta R."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Wang N.S."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Star R.A."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Gonzales P.A."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/author | "Tchapyjnikov D."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/name | "J Am Soc Nephrol"xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/pages | "363-379"xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/title | "Large-scale proteomics and phosphoproteomics of urinary exosomes."xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/19056867 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19056867 |
| http://purl.uniprot.org/citations/19056867 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19056867 |
| http://purl.uniprot.org/uniprot/O95433#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/Q9BQI0#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/Q07075#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/P28838#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/P02647#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/P07741#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |
| http://purl.uniprot.org/uniprot/Q96IU4#attribution-078B3741D2213EB401E4284DFB5E60A3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19056867 |