| http://purl.uniprot.org/citations/19556969 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19556969 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19556969 | http://www.w3.org/2000/01/rdf-schema#comment | "Nonsense-mediated decay (NMD) is a eukaryotic quality control mechanism that degrades mRNAs carrying premature stop codons. In mammalian cells, NMD is triggered when UPF2 bound to UPF3 on a downstream exon junction complex interacts with UPF1 bound to a stalled ribosome. We report structural studies on the interaction between the C-terminal region of UPF2 and intact UPF1. Crystal structures, confirmed by EM and SAXS, show that the UPF1 CH-domain is docked onto its helicase domain in a fixed configuration. The C-terminal region of UPF2 is natively unfolded but binds through separated alpha-helical and beta-hairpin elements to the UPF1 CH-domain. The alpha-helical region binds sixfold more weakly than the beta-hairpin, whereas the combined elements bind 80-fold more tightly. Cellular assays show that NMD is severely affected by mutations disrupting the beta-hairpin binding, but not by those only affecting alpha-helix binding. We propose that the bipartite mode of UPF2 binding to UPF1 brings the ribosome and the EJC in close proximity by forming a tight complex after an initial weak encounter with either element."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2009.175"xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2009.175"xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Sattler M."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Sattler M."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Cusack S."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Cusack S."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Hentze M.W."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Hentze M.W."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Kadlec J."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Kadlec J."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Gutsche I."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Gutsche I."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Clerici M."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Clerici M."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Gehring N.H."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Gehring N.H."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Mourao A."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Mourao A."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Kulozik A."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/author | "Kulozik A."xsd:string |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19556969 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |