| http://purl.uniprot.org/citations/19632001 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19632001 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19632001 | http://www.w3.org/2000/01/rdf-schema#comment | "Centrosomal attachment to nuclei is crucial for proper mitosis and nuclear positioning in various organisms, and generally involves Sun-family proteins located at the inner nuclear envelope. There is still no common scheme for the outer nuclear membrane proteins interacting with Sun1 in centrosome/nucleus attachment. Here we propose a model in which Sun1 mediates a physical link between centrosomes and clustered centromeres through both nuclear membranes in Dictyostelium. For the first time we provide a detailed microscopic analysis of the centrosomal and nuclear envelope localization of endogenous Dictyostelium Sun1 during interphase and mitosis. By immunogold electron microscopy we show that Sun1 is a resident of both nuclear membranes. Disruption of Sun1 function by overexpression of full-length GFP-Sun1 or a GFP-Sun-domain deletion construct revealed not only the established function in centrosome/nucleus attachment and maintenance of ploidy, but also a requirement of Sun1 for the association of the centromere cluster with the centrosome. Live-cell imaging visualized the occurrence of mitotic defects, and demonstrated the requirement of microtubules for dynamic distance changes between centrosomes and nuclei. FRAP analysis revealed at least two populations of Sun1, with an immobile fraction associated with the centrosome, and a mobile fraction in the nuclear envelope."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ejcb.2009.06.003"xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ejcb.2009.06.003"xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Graef R."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Graef R."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Schulz I."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Schulz I."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Baumann O."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Baumann O."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Samereier M."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Samereier M."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Zoglmeier C."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/author | "Zoglmeier C."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/name | "Eur. J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/name | "Eur. J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/pages | "621-638"xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/pages | "621-638"xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/title | "Dictyostelium Sun1 is a dynamic membrane protein of both nuclear membranes and required for centrosomal association with clustered centromeres."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/title | "Dictyostelium Sun1 is a dynamic membrane protein of both nuclear membranes and required for centrosomal association with clustered centromeres."xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/volume | "88"xsd:string |
| http://purl.uniprot.org/citations/19632001 | http://purl.uniprot.org/core/volume | "88"xsd:string |