| http://purl.uniprot.org/citations/19710016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19710016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19710016 | http://www.w3.org/2000/01/rdf-schema#comment | "The Ras/B-Raf/C-Raf/MEK/ERK signaling cascade is critical for the control of many fundamental cellular processes, including proliferation, survival, and differentiation. This study demonstrated that small interfering RNA-dependent knockdown of diacylglycerol kinase eta (DGKeta) impaired the Ras/B-Raf/C-Raf/MEK/ERK pathway activated by epidermal growth factor (EGF) in HeLa cells. Conversely, the overexpression of DGKeta1 could activate the Ras/B-Raf/C-Raf/MEK/ERK pathway in a DGK activity-independent manner, suggesting that DGKeta serves as a scaffold/adaptor protein. By determining the activity of all the components of the pathway in DGKeta-silenced HeLa cells, this study revealed that DGKeta activated C-Raf but not B-Raf. Moreover, this study demonstrated that DGKeta enhanced EGF-induced heterodimerization of C-Raf with B-Raf, which transmits the signal to C-Raf. DGKeta physically interacted with B-Raf and C-Raf, regulating EGF-induced recruitment of B-Raf and C-Raf from the cytosol to membranes. The DGKeta-dependent activation of C-Raf occurred downstream or independently of the already known C-Raf modifications, such as dephosphorylation at Ser-259, phosphorylation at Ser-338, and interaction with 14-3-3 protein. Taken together, the results obtained strongly support that DGKeta acts as a novel critical regulatory component of the Ras/B-Raf/C-Raf/MEK/ERK signaling cascade via a previously unidentified mechanism."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.043604"xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.043604"xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Imai S."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Imai S."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Taketomi A."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Taketomi A."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Yasuda S."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Yasuda S."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Toyota M."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Toyota M."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Kanoh H."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Kanoh H."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Kai M."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Kai M."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Sakane F."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Sakane F."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Takeishi K."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/author | "Takeishi K."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/19710016 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |