http://purl.uniprot.org/citations/19727662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/19727662 | http://www.w3.org/2000/01/rdf-schema#comment | "Aims/hypothesisExtracellular pre-B cell colony-enhancing factor/nicotinamide phosphoribosyltransferase/visfatin (ePBEF/NAMPT/visfatin) is an adipocytokine, whose circulating levels are enhanced in metabolic disorders, such as diabetes mellitus and obesity. Here, we explored the ability of ePBEF/NAMPT/visfatin to promote vascular inflammation, as a condition closely related to atherothrombotic diseases. We specifically studied the ability of PBEF/NAMPT/visfatin to directly activate pathways leading to inducible nitric oxide synthase (iNOS) induction in cultured human aortic smooth muscle cells, as well as the mechanisms involved.MethodsiNOS levels and extracellular signal-regulated kinase (ERK) 1/2 activity were determined by western blotting. Nuclear factor (NF)-kappaB activity was assessed by electrophoretic mobility shift assay.ResultsePBEF/NAMPT/visfatin (10-250 ng/ml) induced iNOS in a concentration-dependent manner. At a submaximal concentration (100 ng/ml), ePBEF/NAMPT/visfatin time-dependently enhanced iNOS levels up to 18 h after stimulation. Over this time period, ePBEF/NAMPT/visfatin elicited a sustained activation of NF-kappaB and triggered a biphasic ERK 1/2 activation. By using the respective ERK 1/2 and NF-kappaB inhibitors, PD98059 and pyrrolidine dithiocarbamate, we established that iNOS induction by ePBEF/NAMPT/visfatin required the consecutive upstream activation of ERK 1/2 and NF-kappaB. The pro-inflammatory action of ePBEF/NAMPT/visfatin was not prevented by insulin receptor blockade. However, exogenous nicotinamide mononucleotide, the product of NAMPT activity, mimicked NF-kappaB activation and iNOS induction by ePBEF/NAMPT/visfatin, while the NAMPT inhibitor APO866 prevented the effects of ePBEF/NAMPT/visfatin on iNOS and NF-kappaB.Conclusions/interpretationThrough its intrinsic NAMPT activity, ePBEF/NAMPT/visfatin appears to be a direct contributor to vascular inflammation, a key feature of atherothrombotic diseases linked to metabolic disorders."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00125-009-1509-2"xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Sanchez-Ferrer C.F."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Nevado J."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Romacho T."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Carraro R."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Cercas E."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Peiro C."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Rodriguez-Manas L."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Azcutia V."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Matesanz N."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/author | "Vazquez-Bella M."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/name | "Diabetologia"xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/pages | "2455-2463"xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/title | "Extracellular PBEF/NAMPT/visfatin activates pro-inflammatory signalling in human vascular smooth muscle cells through nicotinamide phosphoribosyltransferase activity."xsd:string |
http://purl.uniprot.org/citations/19727662 | http://purl.uniprot.org/core/volume | "52"xsd:string |
http://purl.uniprot.org/citations/19727662 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19727662 |
http://purl.uniprot.org/citations/19727662 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19727662 |
http://purl.uniprot.org/uniprot/P01308#attribution-8AA0BD3DA9363A90D453AECA4953E061 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19727662 |
http://purl.uniprot.org/uniprot/P43490#attribution-8AA0BD3DA9363A90D453AECA4953E061 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19727662 |
http://purl.uniprot.org/uniprot/#_Q3V449-mappedCitation-19727662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19727662 |
http://purl.uniprot.org/uniprot/#_Q3V455-mappedCitation-19727662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19727662 |
http://purl.uniprot.org/uniprot/#_Q9EPA7-mappedCitation-19727662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19727662 |