Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/19807924http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19807924http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19807924http://www.w3.org/2000/01/rdf-schema#comment"

Background

Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis. This results in the generation of short intracellular fragments that might either be degraded or translocate to the nucleus to influence transcription. So far, protein-protein interactions that specifically regulate the fate of the intracellular fragments have not been identified.

Results

In order to further define the SH3 domain interactome of the intracellular region of Fas ligand, we now screened a human SH3 domain phage display library. In addition to known SH3 domains mediating binding to the Fas ligand proline-rich domain, we were able to identify a number of additional SH3 domains that might also associate with FasL. Potential functional implications of the new binding proteins for the death factor's biology are discussed. For Tec kinases and sorting nexins, the observed interactions were verified in cellular systems by pulldown experiments.

Conclusion

We provide an extended list of putative Fas ligand interaction partners, confirming previously identified interactions, but also introducing several novel SH3 domain proteins that might be important regulators of Fas ligand function."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.org/dc/terms/identifier"doi:10.1186/1471-2172-10-53"xsd:string
http://purl.uniprot.org/citations/19807924http://purl.org/dc/terms/identifier"doi:10.1186/1471-2172-10-53"xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Voss M."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Voss M."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Janssen O."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Janssen O."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Lettau M."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/author"Lettau M."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/name"BMC Immunol."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/name"BMC Immunol."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/pages"53"xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/pages"53"xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/title"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/title"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/19807924http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/19807924http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19807924
http://purl.uniprot.org/citations/19807924http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19807924
http://purl.uniprot.org/citations/19807924http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19807924
http://purl.uniprot.org/citations/19807924http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19807924
▶