| http://purl.uniprot.org/citations/19892738 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19892738 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19892738 | http://www.w3.org/2000/01/rdf-schema#comment | "Proteolysis has major roles in diverse biologic processes and regulates the activity, localization, and intracellular levels of proteins. Linking signaling pathways and physiologic processes to specific proteolytic processing events is a major challenge in signal transduction research. Here, we describe N-CLAP (N-terminalomics by chemical labeling of the alpha-amine of proteins), a general approach for profiling protein N-termini and identifying protein cleavage sites during cellular signaling. In N-CLAP, simple and readily available reagents are used to selectively affinity label the alpha-amine that characterizes the protein N terminus over the more highly abundant epsilon-amine on lysine residues. Protein cleavage sites are deduced by identifying the corresponding N-CLAP peptides, which are derived from the N-termini of proteins, including the N-termini of the newly formed polypeptide products of proteolytic cleavage. Through selective affinity purification and tandem mass spectrometry analysis of 278 N-CLAP peptides, we characterized proteolytic cleavage events associated with methionine aminopeptidases and signal peptide peptidases, as well as proteins that are proteolytically cleaved after cisplatin-induced apoptosis. Many of the protein cleavage sites that are elicited during apoptotic signaling are consistent with caspase-dependent cleavage. These data demonstrate the utility of N-CLAP for proteomic profiling of protein cleavage sites that are generated during cellular signaling."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0908958106"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0908958106"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Xu G."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Xu G."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Jaffrey S.R."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Jaffrey S.R."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Shin S.B."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/author | "Shin S.B."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/pages | "19310-19315"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/pages | "19310-19315"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/title | "Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/title | "Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini."xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/volume | "106"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://purl.uniprot.org/core/volume | "106"xsd:string |
| http://purl.uniprot.org/citations/19892738 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19892738 |
| http://purl.uniprot.org/citations/19892738 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19892738 |
| http://purl.uniprot.org/citations/19892738 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19892738 |
| http://purl.uniprot.org/citations/19892738 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19892738 |