| http://purl.uniprot.org/citations/19896182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19896182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19896182 | http://www.w3.org/2000/01/rdf-schema#comment | "The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle replication, involves the loading of six related minichromosome maintenance proteins (Mcm2-7) into prereplicative complexes (pre-RCs). Mcm2-7 forms the core of the replicative DNA helicase, which is inactive in the pre-RC. The loading of Mcm2-7 onto DNA requires the origin recognition complex (ORC), Cdc6, and Cdt1, and depends on ATP. We have reconstituted Mcm2-7 loading with purified budding yeast proteins. Using biochemical approaches and electron microscopy, we show that single heptamers of Cdt1*Mcm2-7 are loaded cooperatively and result in association of stable, head-to-head Mcm2-7 double hexamers connected via their N-terminal rings. DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA. Our work has significant implications for understanding how eukaryotic DNA replication origins are chosen and licensed, how replisomes assemble during initiation, and how unwinding occurs during DNA replication."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2009.10.015"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2009.10.015"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Griffith J.D."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Griffith J.D."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Tolun G."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Tolun G."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Morris E.P."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Morris E.P."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Beuron F."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Beuron F."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Diffley J.F."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Diffley J.F."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Remus D."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/author | "Remus D."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/pages | "719-730"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/pages | "719-730"xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/title | "Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing."xsd:string |
| http://purl.uniprot.org/citations/19896182 | http://purl.uniprot.org/core/title | "Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing."xsd:string |