| http://purl.uniprot.org/citations/20704566 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20704566 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20704566 | http://www.w3.org/2000/01/rdf-schema#comment | "The extraordinarily high toxicity of botulinum neurotoxins primarily results from their specific binding and uptake into neurons. At motor neurons, the seven BoNT (botulinum neurotoxin) serotypes A-G inhibit acetylcholine release leading to flaccid paralysis. Uptake of BoNT/A, B, E, F and G requires a dual interaction with gangliosides and the synaptic vesicle proteins synaptotagmin or SV2 (synaptic vesicle glycoprotein 2), whereas little is known about the cell entry mechanisms of the serotypes C and D, which display the lowest amino acid sequence identity compared with the other five serotypes. In the present study we demonstrate that the neurotoxicity of BoNT/D depends on the presence of gangliosides by employing phrenic nerve hemidiaphragm preparations derived from mice expressing the gangliosides GM3, GM2, GM1 and GD1a, or only GM3 [a description of our use of ganglioside nomenclature is given in Svennerholm (1994) Prog. Brain Res. 101, XI-XIV]. High-resolution crystal structures of the 50 kDa cell-binding domain of BoNT/D alone and in complex with sialic acid, as well as biological analyses of single-site BoNT/D mutants identified two carbohydrate-binding sites. One site is located at a position previously identified in BoNT/A, B, E, F and G, but is lacking the conserved SXWY motif. The other site, co-ordinating one molecule of sialic acid, resembles the second ganglioside-binding pocket (the sialic-acid-binding site) of TeNT (tetanus neurotoxin)."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20101042"xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20101042"xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Bigalke H."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Bigalke H."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Binz T."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Binz T."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Lee K."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Lee K."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Jin R."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Jin R."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Mahrhold S."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Mahrhold S."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Pich A."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Pich A."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Rummel A."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Rummel A."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Strotmeier J."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Strotmeier J."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Volker A.K."xsd:string |
| http://purl.uniprot.org/citations/20704566 | http://purl.uniprot.org/core/author | "Volker A.K."xsd:string |