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http://purl.uniprot.org/citations/21276267http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21276267http://www.w3.org/2000/01/rdf-schema#comment"

Background

Transportin-SR2 (TRN-SR2, TNPO3, transportin 3) was previously identified as an interaction partner of human immunodeficiency virus type 1 (HIV-1) integrase and functions as a nuclear import factor of HIV-1. A possible role of capsid in transportin-SR2-mediated nuclear import was recently suggested by the findings that a chimeric HIV virus, carrying the murine leukemia virus (MLV) capsid and matrix proteins, displayed a transportin-SR2 independent phenotype, and that the HIV-1 N74D capsid mutant proved insensitive to transportin-SR2 knockdown.

Results

Our present analysis of viral specificity reveals that TRN-SR2 is not used to the same extent by all lentiviruses. The DNA flap does not determine the TRN-SR2 requirement of HIV-1. We corroborate the TRN-SR2 independent phenotype of the chimeric HIV virus carrying the MLV capsid and matrix proteins. We reanalyzed the HIV-1 N74D capsid mutant in cells transiently or stably depleted of transportin-SR2 and confirm that the N74D capsid mutant is independent of TRN-SR2 when pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G). Remarkably, although somewhat less dependent on TRN-SR2 than wild type virus, the N74D capsid mutant carrying the wild type HIV-1 envelope required TRN-SR2 for efficient replication. By pseudotyping with envelopes that mediate pH-independent viral uptake including HIV-1, measles virus and amphotropic MLV envelopes, we demonstrate that HIV-1 N74D capsid mutant viruses retain partial dependency on TRN-SR2. However, this dependency on TRN-SR2 is lost when the HIV N74D capsid mutant is pseudotyped with envelopes mediating pH-dependent endocytosis, such as the VSV-G and Ebola virus envelopes.

Conclusion

Here we discover a link between the viral entry of HIV and its interaction with TRN-SR2. Our data confirm the importance of TRN-SR2 in HIV-1 replication and argue for careful interpretation of experiments performed with VSV-G pseudotyped viruses in studies on early steps of HIV replication including the role of capsid therein."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.org/dc/terms/identifier"doi:10.1186/1742-4690-8-7"xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Gerard M."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Christ F."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"De Rijck J."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Debyser Z."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Gijsbers R."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Thys W."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Demeulemeester J."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Vancraenenbroeck R."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"De Houwer S."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/author"Taltynov O."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/name"Retrovirology"xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/pages"7"xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/title"Interplay between HIV entry and transportin-SR2 dependency."xsd:string
http://purl.uniprot.org/citations/21276267http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/21276267http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21276267
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http://purl.uniprot.org/uniprot/Q9Y5L0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21276267
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