| http://purl.uniprot.org/citations/21427704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21427704 | http://www.w3.org/2000/01/rdf-schema#comment | "Stromal interaction molecule (STIM1) and ORAI1 are key components of the Ca(2+) release-activated Ca(2+) (CRAC) current having an important role in T-cell activation and mast cell degranulation. CRAC channel activation occurs via physical interaction of ORAI1 with STIM1 when endoplasmic reticulum Ca(2+) stores are depleted. Here we show, utilizing a novel STIM1-derived Förster resonance energy transfer sensor, that the ORAI1 activating small fragment (OASF) undergoes a C-terminal, intramolecular transition into an extended conformation when activating ORAI1. The C-terminal rearrangement of STIM1 does not require a functional CRAC channel, suggesting interaction with ORAI1 as sufficient for this conformational switch. Extended conformations were also engineered by mutations within the first and third coiled-coil domains in the cytosolic portion of STIM1 revealing the involvement of hydrophobic residues in the intramolecular transition. Corresponding full-length STIM1 mutants exhibited enhanced interaction with ORAI1 inducing constitutive CRAC currents, even in the absence of store depletion. We suggest that these mutant STIM1 proteins imitate a physiological activated state, which mimics the intramolecular transition that occurs in native STIM1 upon store depletion."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.79"xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Ikura M."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Romanin C."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Groschner K."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Schindl R."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Stathopulos P."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Fahrner M."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Muik M."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Derler I."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Frischauf I."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Lackner B."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/author | "Plenk P."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/pages | "1678-1689"xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/title | "STIM1 couples to ORAI1 via an intramolecular transition into an extended conformation."xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/21427704 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21427704 |
| http://purl.uniprot.org/citations/21427704 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21427704 |
| http://purl.uniprot.org/uniprot/Q13586#attribution-71DF1BE0F11A1C71A645D88109F29DBB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21427704 |
| http://purl.uniprot.org/uniprot/#_G0XQ39-mappedCitation-21427704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21427704 |
| http://purl.uniprot.org/uniprot/#_Q13586-mappedCitation-21427704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21427704 |
| http://purl.uniprot.org/uniprot/#_Q96D31-mappedCitation-21427704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21427704 |