| http://purl.uniprot.org/citations/21613615 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21613615 | http://www.w3.org/2000/01/rdf-schema#comment | "The posttranscriptional mechanisms whereby RNA-binding proteins (RBPs) regulate T cell differentiation remain unclear. RBPs can coordinately regulate the expression of functionally related genes via binding to shared regulatory sequences, such as the adenylate-uridylate-rich elements (AREs) present in the 3' untranslated region (UTR) of mRNA. The RBP HuR posttranscriptionally regulates IL-4, IL-13, and other Th2 cell-restricted transcripts. We hypothesized that the ARE-bearing GATA-3 gene, a critical regulator of Th2 polarization, is under HuR control as part of its coordinate posttranscriptional regulation of the Th2 program. We report that in parallel with stimulus-induced increase in GATA-3 mRNA and protein levels, GATA-3 mRNA half-life is increased after restimulation in the human T cell line Jurkat, in human memory and Th2 cells, and in murine Th2-skewed cells. We demonstrate by immunoprecipitation of ribonucleoprotein complexes that HuR associates with the GATA-3 endogenous transcript in human T cells and found, using biotin pulldown assay, that HuR specifically interacts with its 3'UTR. Using both loss-of-function and gain-of-function approaches in vitro and in animal models, we show that HuR is a critical mediator of stimulus-induced increase in GATA-3 mRNA and protein expression and that it positively influences GATA-3 mRNA turnover, in parallel with selective promotion of Th2 cytokine overexpression. These results suggest that HuR-driven posttranscriptional control plays a significant role in T cell development and effector function in both murine and human systems. A better understanding of HuR-mediated control of Th2 polarization may have utility in altering allergic airway inflammation in human asthmatic patients."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1001881"xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Fan J."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Fang X."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Atasoy U."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Franklin C.L."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Mori F."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Casolaro V."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Stellato C."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Tartar D.M."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Calaluce R."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Gubin M.M."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Magee J.D."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Dahm G.M."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/author | "Jackson G.A."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/pages | "441-449"xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/title | "Coordinate regulation of GATA-3 and Th2 cytokine gene expression by the RNA-binding protein HuR."xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://purl.uniprot.org/core/volume | "187"xsd:string |
| http://purl.uniprot.org/citations/21613615 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21613615 |
| http://purl.uniprot.org/citations/21613615 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21613615 |
| http://purl.uniprot.org/uniprot/Q15717#attribution-A34C52800F699BB3CE7C8D29C797B1C9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21613615 |