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http://purl.uniprot.org/citations/22001142http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objectives

Tetrandrine (TET), a bisbenzylisoquinoline alkaloid isolated from Stephania tetrandra S. Moore of the Menispermaceae, possesses anti-inflammatory activity. We examined the effect of tetrandrine on interleukin-1β (IL-1β)-provoked inflammatory response in mesangial cells.

Materials and methods

Primary rat mesangial cells (PRMCs) were treated with IL-1β to induce inflammation to resemble glomerulonephritis. Cell viability, morphology and NO production were evaluated. Western blotting was applied for expression of matrix metalloproteinase-9 (MMP-9), inducible NO synthase (iNOS), extracellular signal-regulated kinase (ERK) and NF-κB-related molecules. Electrophoretic mobility shift assay was performed to examine the DNA-binding activity of NF-κB.

Results

TET, at concentrations up to 10 μg/ml, had no significant effect on viability of PRMCs. At non-toxic concentrations, TET inhibited expression of phosphorylated ERK as well as phosphorylated IKK, enhanced degradation of IκBα and reduced the DNA-binding activity of NF-κB in IL-1β-primed PRMCs, suggesting an inhibitory effect on ERK/NF-κB signaling. TET attenuated the IL-1β-provoked expression of iNOS and release of NO. Moreover, both the protein expression and gelatinase activity of MMP-9, but not MMP-2, were markedly suppressed by TET.

Significance

TET down-regulated ERK/NF-κB signaling and inhibited the expression of inflammatory mediators NO and MMP-9. Since these mediators appear to activate mesangial cells, TET may play an important role in prevention of glomerulonephritis."xsd:string
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http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/author"Chen Y.J."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/author"Wu C.J."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/author"Chen H.H."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/author"Wang Y.H."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/author"Lin C.J."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/name"Toxicol In Vitro"xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/pages"1834-1840"xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/title"Tetrandrine down-regulates ERK/NF-kappaB signaling and inhibits activation of mesangial cells."xsd:string
http://purl.uniprot.org/citations/22001142http://purl.uniprot.org/core/volume"25"xsd:string
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