| http://purl.uniprot.org/citations/22003382 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22003382 | http://www.w3.org/2000/01/rdf-schema#comment | "To explore gene therapy strategies for amelogenesis imperfecta (AI), a human ameloblast-like cell population was established from third molars of an AI-affected patient. These cells were characterized by expression of cytokeratin 14, major enamel proteins and alkaline phosphatase staining. Suboptimal transduction of the ameloblast-like cells by an adenovirus type 5 (Ad5) vector was consistent with lower levels of the coxsackie-and-adenovirus receptor (CAR) on those cells relative to CAR-positive A549 cells. To overcome CAR -deficiency, we evaluated capsid-modified Ad5 vectors with various genetic capsid modifications including "pK7" and/or "RGD" motif-containing short peptides incorporated in the capsid protein fiber as well as fiber chimera with the Ad serotype 3 (Ad3) fiber "knob" domain. All fiber modifications provided an augmented transduction of AI-ameloblasts, revealed following vector dose normalization in A549 cells with a superior effect (up to 404-fold) of pK7/RGD double modification. This robust infectivity enhancement occurred through vector binding to both α(v)β3/α(v)β5 integrins and heparan sulfate proteoglycans (HSPGs) highly expressed by AI-ameloblasts as revealed by gene transfer blocking experiments. This work thus not only pioneers establishment of human AI ameloblast-like cell population as a model for in vitro studies but also reveals an optimal infectivity-enhancement strategy for a potential Ad5 vector-mediated gene therapy for AI."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0024281"xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Dong J."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Murakami M."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Wu H."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Ren C."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "MacDougall M."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Lamani E."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Borovjagin A.V."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Passineau M.J."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Keyser E."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/author | "Mamaeva O.A."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/pages | "e24281"xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/title | "Adenovirus gene transfer to amelogenesis imperfecta ameloblast-like cells."xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/22003382 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22003382 |
| http://purl.uniprot.org/citations/22003382 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22003382 |
| http://purl.uniprot.org/uniprot/P78310#attribution-9A37BEA37844815FE79CD36004516AC2 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22003382 |