| http://purl.uniprot.org/citations/22144186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22144186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22144186 | http://www.w3.org/2000/01/rdf-schema#comment | "Tissue factor pathway inhibitor (TFPI) produces factor Xa-dependent feedback inhibition of factor VIIa/tissue factor-induced coagulation. Messages for 2 isoforms of TFPI have been identified. TFPIα mRNA encodes a protein with an acidic N-terminus, 3 Kunitz-type protease inhibitor domains and a basic C-terminus that has been purified from plasma and culture media. TFPIβ mRNA encodes a form in which the Kunitz-3 and C-terminal domains of TFPIα are replaced with an alternative C-terminus that directs the attachment of a glycosylphosphatidylinositol (GPI) anchor, but whether TFPIβ protein is actually expressed is not clear. Moreover, previous studies have suggested that the predominant form of TFPI released from cells by phosphatidylinositol-specific phospholipase C (PIPLC) treatment is TFPIα, implying it is bound at cell surfaces to a separate GPI-anchored coreceptor. Our studies show that the form of TFPI released by PIPLC treatment of cultured endothelial cells and placental microsomes is actually TFPIβ based on (1) migration on SDS-PAGE before and after deglycosylation, (2) the lack of a Kunitz-3 domain, and (3) it contains a GPI anchor. Immunoassays demonstrate that, although endothelial cells secrete TFPIα, greater than 95% of the TFPI released by PIPLC treatment from the surface of endothelial cells and from placental microsomes is TFPIβ."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2011-10-388512"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2011-10-388512"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Broze G.J. Jr."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Broze G.J. Jr."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Girard T.J."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Girard T.J."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Tuley E."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/author | "Tuley E."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/pages | "1256-1262"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/pages | "1256-1262"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/title | "TFPIbeta is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/title | "TFPIbeta is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes."xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/volume | "119"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://purl.uniprot.org/core/volume | "119"xsd:string |
| http://purl.uniprot.org/citations/22144186 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22144186 |
| http://purl.uniprot.org/citations/22144186 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22144186 |
| http://purl.uniprot.org/citations/22144186 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22144186 |
| http://purl.uniprot.org/citations/22144186 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22144186 |