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http://purl.uniprot.org/citations/22187158http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22187158http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22187158http://www.w3.org/2000/01/rdf-schema#comment"Nuclear factor (NF)-κB is a master regulator of pro-inflammatory genes and is upregulated in human immunodeficiency virus 1 (HIV-1) infection. Mechanisms underlying the NF-κB deregulation by HIV-1 are relevant for immune dysfunction in AIDS. We report that in single round HIV-1 infection, or single-pulse PMA stimulation, the HIV-1 Tat transactivator activated NF-κB by hijacking the inhibitor IκB-α and by preventing the repressor binding to the NF-κB complex. Moreover, Tat associated with the p65 subunit of NF-κB and increased the p65 DNA-binding affinity and transcriptional activity. The arginine- and cysteine-rich domains of Tat were required for IκB-α and p65 association, respectively, and for sustaining the NF-κB activity. Among an array of NF-κB-responsive genes, Tat mostly activated the MIP-1α expression in a p65-dependent manner, and bound to the MIP-1α NF-κB enhancer thus promoting the recruitment of p65 with displacement of IκB-α; similar findings were obtained for the NF-κB-responsive genes CSF3, LTA, NFKBIA and TLR2. Our results support a novel mechanism of NF-κB activation via physical interaction of Tat with IκB-α and p65, and may contribute to further insights into the deregulation of the inflammatory response by HIV-1."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.org/dc/terms/identifier"doi:10.1093/nar/gkr1224"xsd:string
http://purl.uniprot.org/citations/22187158http://purl.org/dc/terms/identifier"doi:10.1093/nar/gkr1224"xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Rossi A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Rossi A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Falcone C."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Falcone C."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Scialdone A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Scialdone A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Fiume G."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Fiume G."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"De Laurentiis A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"De Laurentiis A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Fasanella Masci F."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Fasanella Masci F."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Palmieri C."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Palmieri C."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Pisano A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Pisano A."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Pontoriero M."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Pontoriero M."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Quinto I."xsd:string
http://purl.uniprot.org/citations/22187158http://purl.uniprot.org/core/author"Quinto I."xsd:string
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