| http://purl.uniprot.org/citations/22245611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22245611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22245611 | http://www.w3.org/2000/01/rdf-schema#comment | "Drought is one of the most important limiting factors in crop production. To identify genes required for the drought stress response in the cereal crop maize, a gene coding for RING-finger protein (ZmRFP1), which is highly responsive to PEG-induced drought stress, was isolated by mRNA differential display and rapid amplification of cDNA ends. The ZmRFP1 encodes a protein of 280 amino acids and contains a single C(3)H(2)C(3)-type RING motif in its C-terminal region. ZmRFP1 is an ortholog of Arabidopsis SDIR1 (salt- and drought-induced RING finger 1) (66% identity to AtSDIR1).The recombinant ZmRFP1 protein purified from Escherichia coli exhibited an in vitro E3 ubiquitin ligase activity. Real-time PCR analysis indicates that the transcript levels of ZmRFP1 were higher in aerial tissues including stems, leaves, tassels and immature ears, and were markedly up-regulated by drought stress, and exogenous ABA, but not by salt, heat and cold stresses. Transient expression of the green fluorescent protein (GFP)-ZmRFP1 fusion protein in onion cells revealed a plasma membrane localization of the protein. Further analysis of ZmRFP1 transcripts between an ABA-deficient transposon mutant viviparous14 (vp14) and its isogenic wild-type line W22 showed that ZmRFP1 transcript levels were induced significantly in the wild-type line under drought stress, but not in the mutant line VP14. These results indicate that ZmRFP1 responds to drought stress in an ABA-dependent way and is likely to function in the ubiquitin conjunction pathway. The ZmRFP1 might serve as a candidate gene in genetic improvement for drought tolerance engineering in cereal crop plants."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2011.12.028"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2011.12.028"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Ding J."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Ding J."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Wu J."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Wu J."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Xia Z."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/author | "Xia Z."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/pages | "146-153"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/pages | "146-153"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/title | "ZmRFP1, the putative ortholog of SDIR1, encodes a RING-H2 E3 ubiquitin ligase and responds to drought stress in an ABA-dependent manner in maize."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/title | "ZmRFP1, the putative ortholog of SDIR1, encodes a RING-H2 E3 ubiquitin ligase and responds to drought stress in an ABA-dependent manner in maize."xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/volume | "495"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://purl.uniprot.org/core/volume | "495"xsd:string |
| http://purl.uniprot.org/citations/22245611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22245611 |
| http://purl.uniprot.org/citations/22245611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22245611 |