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http://purl.uniprot.org/citations/23665276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23665276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23665276 | http://www.w3.org/2000/01/rdf-schema#comment | "Background & aimsShort-chain fatty acids (SCFAs), the most abundant microbial metabolites in the intestine, activate cells via G-protein-coupled receptors (GPRs), such as GPR41 and GPR43. We studied regulation of the immune response by SCFAs and their receptors in the intestines of mice.MethodsInflammatory responses were induced in GPR41(-/-), GPR43(-/-), and C57BL6 (control) mice by administration of ethanol; 2, 4, 6-trinitrobenzene sulfonic-acid (TNBS); or infection with Citrobacter rodentium. We examined the effects of C rodentium infection on control mice fed SCFAs and/or given injections of antibodies that delay the immune response. We also studied the kinetics of cytokine and chemokine production, leukocyte recruitment, intestinal permeability, and T-cell responses. Primary colon epithelial cells were isolated from GPR41(-/-), GPR43(-/-), and control mice; signaling pathways regulated by SCFAs were identified using immunohistochemical, enzyme-linked immunosorbent assay, and flow cytometry analyses.ResultsGPR41(-/-) and GPR43(-/-) mice had reduced inflammatory responses after administration of ethanol or TNBS compared with control mice, and had a slower immune response against C rodentium infection, clearing the bacteria more slowly. SCFAs activated intestinal epithelial cells to produce chemokines and cytokines in culture and mice after administration of ethanol, TNBS, or C rodentium. These processes required GPR41 and GPR43 and were required to recruit leukocytes and activate effector T cells in the intestine. GPR41 and GPR43 activated extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling pathways in epithelial cells to induce production of chemokines and cytokines during immune responses.ConclusionsSCFAs activate GPR41 and GPR43 on intestinal epithelial cells, leading to mitogen-activated protein kinase signaling and rapid production of chemokines and cytokines. These pathways mediate protective immunity and tissue inflammation in mice."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.org/dc/terms/identifier | "doi:10.1053/j.gastro.2013.04.056"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.org/dc/terms/identifier | "doi:10.1053/j.gastro.2013.04.056"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kim C.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kim C.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Park J.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Park J.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Yanagisawa M."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Yanagisawa M."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kang S.G."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kang S.G."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kim M.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/author | "Kim M.H."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/name | "Gastroenterology"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/name | "Gastroenterology"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/pages | "396-406"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/pages | "396-406"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/title | "Short-chain fatty acids activate GPR41 and GPR43 on intestinal epithelial cells to promote inflammatory responses in mice."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/title | "Short-chain fatty acids activate GPR41 and GPR43 on intestinal epithelial cells to promote inflammatory responses in mice."xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/volume | "145"xsd:string |
http://purl.uniprot.org/citations/23665276 | http://purl.uniprot.org/core/volume | "145"xsd:string |