| http://purl.uniprot.org/citations/23821748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23821748 | http://www.w3.org/2000/01/rdf-schema#comment | "Membrane fusion is mediated by complexes formed by SNAP-receptor (SNARE) and Secretory 1 (Sec1)/mammalian uncoordinated-18 (Munc18)-like (SM) proteins, but it is unclear when and how these complexes assemble. Here we describe an improved two-color fluorescence nanoscopy technique that can achieve effective resolutions of up to 7.5-nm full width at half maximum (3.2-nm localization precision), limited only by stochastic photon emission from single molecules. We use this technique to dissect the spatial relationships between the neuronal SM protein Munc18-1 and SNARE proteins syntaxin-1 and SNAP-25 (25 kDa synaptosome-associated protein). Strikingly, we observed nanoscale clusters consisting of syntaxin-1 and SNAP-25 that contained associated Munc18-1. Rescue experiments with syntaxin-1 mutants revealed that Munc18-1 recruitment to the plasma membrane depends on the Munc18-1 binding to the N-terminal peptide of syntaxin-1. Our results suggest that in a primary neuron, SNARE/SM protein complexes containing syntaxin-1, SNAP-25, and Munc18-1 are preassembled in microdomains on the presynaptic plasma membrane. Our superresolution imaging method provides a framework for investigating interactions between the synaptic vesicle fusion machinery and other subcellular systems in situ."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1310654110"xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Chu S."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Brunger A.T."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Sharma M."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Sudhof T.C."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Park S.R."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Pang Z.P."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Mukherjee K."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/author | "Pertsinidis A."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/pages | "E2812-20"xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/title | "Ultrahigh-resolution imaging reveals formation of neuronal SNARE/Munc18 complexes in situ."xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://purl.uniprot.org/core/volume | "110"xsd:string |
| http://purl.uniprot.org/citations/23821748 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23821748 |
| http://purl.uniprot.org/citations/23821748 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23821748 |
| http://purl.uniprot.org/uniprot/P60879#attribution-0FBB572EF10BC865FC5CB6974C425F11 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23821748 |
| http://purl.uniprot.org/uniprot/P61264#attribution-0FBB572EF10BC865FC5CB6974C425F11 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23821748 |
| http://purl.uniprot.org/uniprot/O35526#attribution-0FBB572EF10BC865FC5CB6974C425F11 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23821748 |
| http://purl.uniprot.org/uniprot/O08599#attribution-0FBB572EF10BC865FC5CB6974C425F11 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23821748 |
| http://purl.uniprot.org/uniprot/O08599#attribution-43CAFB6063F3A85624B8F82DB9AC0F96 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23821748 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YUA0-mappedCitation-23821748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23821748 |