| http://purl.uniprot.org/citations/2430793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2430793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2430793 | http://www.w3.org/2000/01/rdf-schema#comment | "A human endothelial cDNA expression library, based on the Escherichia coli plasmid pUC9, was screened with a heterologous antibody raised against purified bovine aortic endothelial plasminogen activator inhibitor (PAI). A synthetic oligonucleotide, derived from a partial PAI cDNA expression clone, was used to select a full-length PAI cDNA, the size of which coincides with the length of PAI mRNA (approximately 2350 nucleotides) as determined by Northern blot analysis. The authenticity of full-length PAI cDNA is demonstrated by the expression of biologically active PAI both in lysates of transformed E. coli cells and in conditioned media of mouse Ltk-cells, transfected with PAI cDNA inserted into vector pSV2. Analysis of the de novo synthesized anti-plasminogen activator activity, employing reverse fibrin autography, shows that transfected mouse Ltk-cells synthesize a polypeptide with a mol. wt identical to that of the native PAI glycoprotein (Mr 52,000), whereas in E. coli an unglycosylated, active product with a mol. wt of 43,000 is made. The amino acid sequence, derived from the determined nucleotide sequence, shows that pre-PAI consists of 402 amino acids. It is proposed that the mature PAI is preceded by a signal peptide of 23 amino acid residues. The amino acid sequence of mature PAI includes three potential asparagine-linked glycosylation sites and lacks cysteine residues. The predicted amino acid sequence reveals significant homology with members of the serine protease inhibitor (Serpin) family, e.g. alpha 1-proteinase inhibitor and antithrombin III.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1986.tb04532.x"xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1986.tb04532.x"xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Pannekoek H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Pannekoek H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Diergaarde P."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Diergaarde P."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Lambers H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Lambers H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "van Mourik J.A."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "van Mourik J.A."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Verweij C.L."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Verweij C.L."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Veerman H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "Veerman H."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "van Zonneveld A.-J."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/author | "van Zonneveld A.-J."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/pages | "2539-2544"xsd:string |
| http://purl.uniprot.org/citations/2430793 | http://purl.uniprot.org/core/pages | "2539-2544"xsd:string |