Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/25217442http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25217442http://www.w3.org/2000/01/rdf-schema#comment"

Rationale

Many processes in endothelial cells including angiogenic responses are regulated by microRNAs. However, there is limited information available about their complex cross-talk in regulating certain endothelial functions.

Aim

The objective of this study is to identify endothelial functions of the pro-hypertrophic miR-212/132 cluster and its cross-talk with other microRNAs during development and disease.

Methods and results

We here show that anti-angiogenic stimulation by transforming growth factor-beta activates the microRNA-212/132 cluster by derepression of their transcriptional co-activator cAMP response element-binding protein (CREB)-binding protein (CBP) which is a novel target of a previously identified pro-angiogenic miRNA miR-30a-3p in endothelial cells. Surprisingly, despite having the same seed-sequence, miR-212 and miR-132 exerted differential effects on endothelial transcriptome regulation and cellular functions with stronger endothelial inhibitory effects caused by miR-212. These differences could be attributed to additional auxiliary binding of miR-212 to its targets. In vivo, deletion of the miR-212/132 cluster increased endothelial vasodilatory function, improved angiogenic responses during postnatal development and in adult mice.

Conclusion

Our results identify (i) a novel miRNA-cross-talk involving miR-30a-3p and miR-212, which led to suppression of important endothelial genes such as GAB1 and SIRT1 finally culminating in impaired endothelial function; and (ii) microRNAs may have different biological roles despite having the same seed sequence."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.org/dc/terms/identifier"doi:10.1093/eurheartj/ehu344"xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Gupta S.K."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Chowdhury K."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Ucar A."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Batkai S."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Thum T."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Napp L.C."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Beermann J."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Lorenzen J.M."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Melk A."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Volkmann I."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Kumarswamy R."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Bhayadia R."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/author"Jabs O."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/name"Eur Heart J"xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/pages"3224-3231"xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/title"Vascular importance of the miR-212/132 cluster."xsd:string
http://purl.uniprot.org/citations/25217442http://purl.uniprot.org/core/volume"35"xsd:string
http://purl.uniprot.org/citations/25217442http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25217442
http://purl.uniprot.org/citations/25217442http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25217442
http://purl.uniprot.org/uniprot/Q13480#attribution-AEA057E537B9788BA578145BBFC105E6http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25217442
http://purl.uniprot.org/uniprot/Q96EB6#attribution-BAA8CE6978FCFE452AB7D4881445C6E3http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25217442
▶