| http://purl.uniprot.org/citations/25543257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25543257 | http://www.w3.org/2000/01/rdf-schema#comment | "Determining the structures of amyloid fibrils is an important first step toward understanding the molecular basis of neurodegenerative diseases. For β-amyloid (Aβ) fibrils, conventional solid-state NMR structure determination using uniform labeling is limited by extensive peak overlap. We describe the characterization of a distinct structural polymorph of Aβ using solid-state NMR, transmission electron microscopy (TEM), and Rosetta model building. First, the overall fibril arrangement is established using mass-per-length measurements from TEM. Then, the fibril backbone arrangement, stacking registry, and "steric zipper" core interactions are determined using a number of solid-state NMR techniques on sparsely (13)C-labeled samples. Finally, we perform Rosetta structure calculations with an explicitly symmetric representation of the system. We demonstrate the power of the hybrid Rosetta/NMR approach by modeling the in-register, parallel "Iowa" mutant (D23N) at high resolution (1.2Å backbone rmsd). The final models are validated using an independent set of NMR experiments that confirm key features."xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2014.10.022"xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/author | "Yau W.M."xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/author | "Sgourakis N.G."xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/author | "Qiang W."xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/pages | "216-227"xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/title | "Modeling an in-register, parallel "iowa" abeta fibril structure using solid-state NMR data from labeled samples with rosetta."xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/25543257 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25543257 |
| http://purl.uniprot.org/citations/25543257 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25543257 |
| http://purl.uniprot.org/uniprot/P05067#attribution-35325568BFCCB9230BEEAB93C33D6B26 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/25543257 |
| http://purl.uniprot.org/uniprot/#_P05067-mappedCitation-25543257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25543257 |
| http://purl.uniprot.org/uniprot/P05067 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25543257 |