| http://purl.uniprot.org/citations/2665813 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2665813 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2665813 | http://www.w3.org/2000/01/rdf-schema#comment | "We have carried out an extensive protein-protein cross-linking study on the 50S ribosomal subunit of Escherichia coli using four different cross-linking reagents of varying length and specificity. For the unambiguous identification of the members of the cross-linked protein complexes, immunoblotting techniques using antisera specific for each individual ribosomal protein have been used, and for each cross-link, the cross-linking yield has been determined. With the smallest cross-linking reagent diepoxybutane (4 A), four cross-links have been identified, namely, L3-L19, L10-L11, L13-L21, and L14-L19. With the sulfhydryl-specific cross-linking reagent o-phenylenedimaleimide (5.2 A) and p-phenylenedimaleimide (12 A), the cross-links L2-L9, L3-L13, L3-L19, L9-L28, L13-L20, L14-L19, L16-L27, L17-L32, and L20-L21 were formed; in addition, the cross-link L23-L29 was exclusively found with the shorter o-phenylenedimaleimide. The cross-links obtained with dithiobis(succinimidyl propionate) (12 A) were L1-L33, L2-L9, L2-L9-L28, L3-L19, L9-L28, L13-L21, L14-L19, L16-L27, L17-L32, L19-L25, L20-L21, and L23-L34. The good agreement of the cross-links obtained with the different cross-linking reagents used in this study demonstrates the reliability of our cross-linking approach. Incorporation of our cross-linking results into the three-dimensional model of the 50S ribosomal subunit derived from immunoelectron microscopy yields the locations for 29 of the 33 proteins within the larger ribosomal subunit."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00435a071"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00435a071"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Redl B."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Redl B."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Stoeffler G."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Stoeffler G."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Martin T."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Martin T."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Stoeffler-Meilicke M."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Stoeffler-Meilicke M."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Walleczek J."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/author | "Walleczek J."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/pages | "4099-4105"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/pages | "4099-4105"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/title | "Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/title | "Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/2665813 | http://purl.uniprot.org/core/volume | "28"xsd:string |