| http://purl.uniprot.org/citations/26950439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26950439 | http://www.w3.org/2000/01/rdf-schema#comment | "Cystic fibrosis results from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent protein kinase A (PKA) and ATP-regulated chloride channel. Here, we demonstrate that nucleoside diphosphate kinase B (NDPK-B, NM23-H2) forms a functional complex with CFTR. In airway epithelia forskolin/IBMX significantly increases NDPK-B co-localisation with CFTR whereas PKA inhibitors attenuate complex formation. Furthermore, an NDPK-B derived peptide (but not its NDPK-A equivalent) disrupts the NDPK-B/CFTR complex in vitro (19-mers comprising amino acids 36-54 from NDPK-B or NDPK-A). Overlay (Far-Western) and Surface Plasmon Resonance (SPR) analysis both demonstrate that NDPK-B binds CFTR within its first nucleotide binding domain (NBD1, CFTR amino acids 351-727). Analysis of chloride currents reflective of CFTR or outwardly rectifying chloride channels (ORCC, DIDS-sensitive) showed that the 19-mer NDPK-B peptide (but not its NDPK-A equivalent) reduced both chloride conductances. Additionally, the NDPK-B (but not NDPK-A) peptide also attenuated acetylcholine-induced intestinal short circuit currents. In silico analysis of the NBD1/NDPK-B complex reveals an extended interaction surface between the two proteins. This binding zone is also target of the 19-mer NDPK-B peptide, thus confirming its capability to disrupt NDPK-B/CFTR complex. We propose that NDPK-B forms part of the complex that controls chloride currents in epithelia."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0149097"xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Mehta A."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Taylor C.J."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Lascu I."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Kerbiriou M."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Trouve P."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Borthwick L.A."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Robson L."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Cozza G."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Postel E.H."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Cassidy D."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/author | "Muimo R."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/pages | "e0149097"xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/title | "Role of Interaction and Nucleoside Diphosphate Kinase B in Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Function by cAMP-Dependent Protein Kinase A."xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/26950439 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26950439 |
| http://purl.uniprot.org/citations/26950439 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26950439 |
| http://purl.uniprot.org/uniprot/#_A0A089NFM8-mappedCitation-26950439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26950439 |
| http://purl.uniprot.org/uniprot/#_A0A089PPW6-mappedCitation-26950439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26950439 |
| http://purl.uniprot.org/uniprot/#_A0A088T1A1-mappedCitation-26950439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26950439 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB9-mappedCitation-26950439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26950439 |