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http://purl.uniprot.org/citations/2753890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2753890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2753890http://www.w3.org/2000/01/rdf-schema#comment"In the small intestine, proglucagon is processed into the previously characterized peptide "glicentin" (proglucagon (PG) 1-69) and two smaller peptides showing about 50% homology with glucagon: glucagon-like peptide-1 and -2. It was assumed that the sites of post-translational cleavage in the small intestine of the proglucagon precursor were determined by pairs of basic amino acid residues flanking the two peptides. Earlier studies have shown that synthetic glucagon-like peptide-1 (GLP-1) synthesized according to the proposed structure (proglucagon 71-108 or because residue 108 is Gly, 72-107 amide) had no physiological effects, whereas a truncated from of GLP-1, corresponding to proglucagon 78-107 amide, strongly stimulated insulin secretion and depressed glucagon secretion. To determine the amino acid sequence of the naturally occurring peptide we isolated GLP-1 from human small intestine by hydrophobic, gel permeation, and reverse-phase high performance liquid chromatography. By analysis of composition and sequence it was determined that the peptide corresponded to PG 78-107. By mass spectrometry the molecular mass was determined to be 3295, corresponding to PG 78-107 amide. Furthermore, mass spectrometry of the methyl-esterified peptide showed an increase in mass compatible with the presence of alpha-carboxyl amidation. Thus, the gut-derived insulinotrophic hormone GLP-1 is shown to be PG 78-107 amide."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)51561-1"xsd:string
http://purl.uniprot.org/citations/2753890http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)51561-1"xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Hoejrup P."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Hoejrup P."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Johnsen A.H."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Johnsen A.H."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Bersani M."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Bersani M."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Holst J.J."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Holst J.J."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Orskov C."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/author"Orskov C."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/date"1989"xsd:gYear
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/date"1989"xsd:gYear
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/pages"12826-12829"xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/pages"12826-12829"xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/title"Complete sequences of glucagon-like peptide-1 from human and pig small intestine."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/title"Complete sequences of glucagon-like peptide-1 from human and pig small intestine."xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/volume"264"xsd:string
http://purl.uniprot.org/citations/2753890http://purl.uniprot.org/core/volume"264"xsd:string