| http://purl.uniprot.org/citations/28124060 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28124060 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNAs are small non-coding RNAs that negatively regulate posttranscriptional gene expression. Several microRNAs have been described to regulate the process of angiogenesis. Previously, we have shown that bone morphogenetic protein 4 (BMP4) increased the pro-angiogenic activity of endothelial cells. In this project, we now investigated how the pro-angiogenic BMP4 effect is mediated by microRNAs. First, we performed a microRNA array with BMP4-stimulated human umbilical vein endothelial cells (HUVECs). Among the top-regulated microRNAs, we detected a decreased expression of miR-494 and increased expression of miR-126-5p. Next, we analysed the canonical Smad and alternative signalling pathways, through which BMP4 would regulate miR-126-5p and miR-494 expression. Furthermore, the functional effect of miR-494 and miR-126-5p on endothelial cells was investigated. MicroRNA-494 overexpression decreased endothelial cell proliferation, migration and sprout formation. Consistently, miR-494 inhibition increased endothelial cell function. As potential miR-494 targets, bFGF and BMP endothelial cell precursor-derived regulator (BMPER) were identified and confirmed by western blot. Luciferase assays showed direct miR-494 binding in BMPER 3'UTR. In contrast, miR-126-5p overexpression increased pro-angiogenic endothelial cell behaviour and, accordingly, miR-126-5p inhibition decreased endothelial cell function. As a direct miR-126-5p target we identified the anti-angiogenic thrombospondin-1 which was confirmed by western blot analysis and luciferase assays. In the Matrigel plug assay application of antagomiR-494 increased endothelial cell ingrowth, whereas antagomiR-126-5p treatment decreased cell ingrowth in vivo. Taken together, through differential regulation of the anti-angiomiR-494 and the angiomiR-126-5p by BMP4 both microRNAs contribute to the pro-angiogenic BMP4 effect on endothelial cells."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.org/dc/terms/identifier | "doi:10.1160/th16-08-0643"xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Zhou Q."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Moser M."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Bode C."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Grundmann S."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Engert B."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Esser J.S."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Pankratz F."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/author | "Saretzki E."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/name | "Thromb Haemost"xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/pages | "734-749"xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/title | "Bone morphogenetic protein 4 regulates microRNAs miR-494 and miR-126-5p in control of endothelial cell function in angiogenesis."xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/28124060 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28124060 |
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| http://purl.uniprot.org/uniprot/P18075#attribution-D4434E1044313E16DDF0170E659623DB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28124060 |
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