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http://purl.uniprot.org/citations/28595095http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28595095http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To compare the expression patterns of two multiple transcripts derived from DSP-PP gene during tooth development. One is DSP-only transcript (i.e. does not encode PP) and the other is DSP-PP523 transcript, a main DSP-PP transcript.

Design

Unique antisense and sense riboprobes were generated from DSP-only and DSPPP523 cDNAs for in situ studies to examine DSP-only and DSP-PP523 transcript expression in developing molars. Paraffin-embedded sections (5-7μ m) from embryonic 20day, postnatal 2, 3 and 6days were deparaffined and hydrated. Tissues were prehybridized, then hybridized with DSP-only and DSP-PP523 anti-sense (AS) or sense (S) Digoxigenin labeled-riboprobes overnight, and washed. Anti-Digoxigenin antibodies conjugated to alkaline phosphatase were used to detect the presence of bound riboprobes by color reaction with NBT/BCIP. Stro-1 antibody was used for immunohistochemical analysis of Stro-1 protein expression in rat molars.

Results

We found that unlike the DSP-PP523 transcript, the DSP-only transcript does not express in the entire polarized mature odontoblasts but is expressed in the areas subjacent to the mature odontoblast layer. In addition, DSP-only transcript is expressed in the dental pulp. Interestingly, Stro-1 protein, a stem cell marker, was also identified in the areas subjacentto odontoblasts and in dental pulp.

Conclusion

Differential expression of DSP-only and DSP-PP523 transcripts suggest that these two kinds of transcripts may play different roles during dentinogenesis. DSP-PP523 transcript is expressed in mature odontoblasts, which actively participates in dentin formation. DSP-only transcript might have a different function."xsd:string
http://purl.uniprot.org/citations/28595095http://purl.org/dc/terms/identifier"doi:10.1016/j.archoralbio.2017.04.031"xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/author"Zhu Y.Q."xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/author"Ritchie H.H."xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/author"Song R.M."xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/name"Arch Oral Biol"xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/pages"33-37"xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/title"Differential expression between "DSP-only" and DSP-PP523 transcripts in rat molar teeth."xsd:string
http://purl.uniprot.org/citations/28595095http://purl.uniprot.org/core/volume"82"xsd:string
http://purl.uniprot.org/citations/28595095http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28595095
http://purl.uniprot.org/citations/28595095http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/28595095
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