| http://purl.uniprot.org/citations/28741290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28741290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28741290 | http://www.w3.org/2000/01/rdf-schema#comment | "Aminoacyl-tRNA synthetases (aaRSs) play essential roles in protein synthesis. As a member of the aaRS family, the tyrosyl-tRNA synthetase (TyrRS) in Escherichia coli has been shown in proteomic studies to be acetylated at multiple lysine residues. However, these putative acetylation targets have not yet been biochemically characterized. In this study, we applied a genetic-code-expansion strategy to site-specifically incorporate Nϵ -acetyl-l-lysine into selected positions of TyrRS for in vitro characterization. Enzyme assays demonstrated that acetylation at K85, K235, and K238 could impair the enzyme activity. In vitro deacetylation experiments showed that most acetylated lysine residues in TyrRS were sensitive to the E. coli deacetylase CobB but not YcgC. In vitro acetylation assays indicated that 25 members of the Gcn5-related N-acetyltransferase family in E. coli, including YfiQ, could not acetylate TyrRS efficiently, whereas TyrRS could be acetylated chemically by acetyl-CoA or acetyl-phosphate (AcP) only. Our in vitro characterization experiments indicated that lysine acetylation could be a possible mechanism for modulating aaRS enzyme activities, thus affecting translation."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.201700343"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.201700343"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Fan C."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Fan C."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Gan Q."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Gan Q."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Gregory C."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Gregory C."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Venkat S."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/author | "Venkat S."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/pages | "1928-1934"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/pages | "1928-1934"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/title | "Biochemical characterization of the lysine acetylation of tyrosyl-tRNA synthetase in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/title | "Biochemical characterization of the lysine acetylation of tyrosyl-tRNA synthetase in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/28741290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28741290 |
| http://purl.uniprot.org/citations/28741290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28741290 |