| http://purl.uniprot.org/citations/2954953 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2954953 | http://www.w3.org/2000/01/rdf-schema#comment | "The receptor for human interferon-gamma (IFN-gamma) was purified from foreskin fibroblasts. Triton X-100 extracts obtained from either intact cells or membrane preparations were passed through an immobilized interferon-gamma column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of eluted fractions revealed a major band of Mr = 95,000 and minor bands of Mr = 80,000 and 60,000. Further purification was obtained by steric exclusion and by lectin chromatography. The purified receptor retained the ability to bind 125I-IFN-gamma with a Kd of 2.2 X 10(-10) M, a value close to that obtained with intact fibroblasts (5 X 10(-10) M). A complex of Mr = 105,000-125,000 was visualized by immunoprecipitation of 125I-IFN-gamma cross-linked to the purified receptor followed by SDS-PAGE and autoradiography. A similar complex was obtained when 125I-IFN-gamma was cross-linked to intact cells. Immunization of mice with the excised SDS-PAGE band of Mr = 95,000 elicited antibodies that blocked the antiviral activity of IFN-gamma and immunoprecipitated the cross-linked complex of 125I-IFN-gamma and its receptor."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)47439-x"xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/author | "Revel M."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/author | "Novick D."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/author | "Rubinstein M."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/author | "Orchansky P."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/pages | "8483-8487"xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/title | "The human interferon-gamma receptor. Purification, characterization, and preparation of antibodies."xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://purl.uniprot.org/core/volume | "262"xsd:string |
| http://purl.uniprot.org/citations/2954953 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2954953 |
| http://purl.uniprot.org/citations/2954953 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2954953 |
| http://purl.uniprot.org/uniprot/P15260#attribution-BA8D778F80EA8ABE75CFB6CD01FC84A1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2954953 |