| http://purl.uniprot.org/citations/3034864 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3034864 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3034864 | http://www.w3.org/2000/01/rdf-schema#comment | "A gene bank was constructed from Pseudomonas aeruginosa PAO1 and used to complement three P. aeruginosa elastase-deficient strains. One clone, pRF1, contained a gene which restored elastase production in two P. aeruginosa isolates deficient in elastase production (PA-E15 and PAO-E105). This gene also encoded production of elastase antigen and activity in Escherichia coli and is the structural gene for Pseudomonas elastase. A second clone, pHN13, contained a 20-kilobase (kb) EcoRI insert which was not related to the 8-kb EcoRI insert of pRF1 as determined by restriction analysis and DNA hybridization. A 2.2-kb SalI-HindIII fragment from pHN3 was subcloned into pUC18, forming pRB1822-1. Plasmid pRB1822-1 restored normal elastolytic activity to PAO-E64, a mutant for elastase activity. Clones derived from pHN13 failed to elicit elastase antigen or enzymatic activity in E. coli."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.169.6.2691-2696.1987"xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.169.6.2691-2696.1987"xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Nicas T.I."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Nicas T.I."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Iglewski B.H."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Iglewski B.H."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Bever R.A."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Bever R.A."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Hanne L.F."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Hanne L.F."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Leduc F."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Leduc F."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Schad P.A."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/author | "Schad P.A."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/pages | "2691-2696"xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/pages | "2691-2696"xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/title | "Cloning and characterization of elastase genes from Pseudomonas aeruginosa."xsd:string |
| http://purl.uniprot.org/citations/3034864 | http://purl.uniprot.org/core/title | "Cloning and characterization of elastase genes from Pseudomonas aeruginosa."xsd:string |