| http://purl.uniprot.org/citations/31230944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31230944 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, are largely unclear. Here, we unravel the structural basis and mechanistic consequences of a conserved autoubiquitination event near the catalytic center of E2s, using the human anaphase-promoting complex/cyclosome-associated UBE2S as a model system. Crystal structures we determined of the catalytic ubiquitin carrier protein domain combined with MD simulations reveal that the active-site region is malleable, which permits an adjacent ubiquitin acceptor site, Lys+5, to be ubiquitinated intramolecularly. We demonstrate by NMR that the Lys+5-linked ubiquitin inhibits UBE2S by obstructing its reloading with ubiquitin. By immunoprecipitation, quantitative mass spectrometry, and siRNA-and-rescue experiments we show that Lys+5 ubiquitination of UBE2S decreases during mitotic exit but does not influence proteasomal turnover of this E2. These findings suggest that UBE2S activity underlies inherent regulation during the cell cycle."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2019.05.008"xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Yu L."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Schweimer K."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Urlaub H."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Choudhary J.S."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Lorenz S."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Diebold M."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Mansfeld J."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Dybkov O."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Roumeliotis T.I."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Sotriffer C."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Kucerova A."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/author | "Liess A.K.L."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/pages | "1195-1210.e7"xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/title | "Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination."xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/31230944 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31230944 |
| http://purl.uniprot.org/citations/31230944 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31230944 |
| http://purl.uniprot.org/uniprot/#_Q2QD04-mappedCitation-31230944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31230944 |
| http://purl.uniprot.org/uniprot/#_Q16763-mappedCitation-31230944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31230944 |
| http://purl.uniprot.org/uniprot/#_Q6NXQ4-mappedCitation-31230944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31230944 |