| http://purl.uniprot.org/citations/3264877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3264877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3264877 | http://www.w3.org/2000/01/rdf-schema#comment | "The biosynthesis of macrophage colony-stimulating factor 1 (CSF-1) was examined in mouse NIH-3T3 fibroblasts transfected with a retroviral vector expressing the 554-amino-acid product of a human 4-kilobase (kb) CSF-1 cDNA. Similar to results previously obtained with a 1.6-kb human cDNA that codes for a 256-amino-acid CSF-1 precursor, the results of the present study showed that NIH-3T3 cells expressing the product of the 4-kb clone produced biologically active human CSF-1 and were transformed by an autocrine mechanism when cotransfected with a vector containing a human c-fms (CSF-1 receptor) cDNA. The 4-kb CSF-1 cDNA product was synthesized as an integral transmembrane glycoprotein that was assembled into disulfide-linked dimers and rapidly underwent proteolytic cleavage to generate a soluble growth factor. Although the smaller CSF-1 precursor specified by the 1.6-kb human cDNA was stably expressed as a membrane-bound glycoprotein at the cell surface and was slowly cleaved to release the extracellular growth factor, the cell-associated product of the 4-kb clone was efficiently processed to the secreted form and was not detected on the plasma membrane. Digestion with glycosidic enzymes indicated that soluble CSF-1 encoded by the 4-kb cDNA contained both asparagine(N)-linked and O-linked carbohydrate chains, whereas the product of the 1.6-kb clone had only N-linked oligosaccharides. Removal of the carbohydrate indicated that the polypeptide chain of the secreted 4-kb cDNA product was longer than that of the corresponding form encoded by the smaller clone. These differences in posttranslational processing may reflect diverse physiological roles for the products of the two CSF-1 precursors in vivo."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.8.11.5026-5034.1988"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.8.11.5026-5034.1988"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/author | "Roussel M.F."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/author | "Roussel M.F."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/author | "Rettenmier C.W."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/author | "Rettenmier C.W."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/pages | "5026-5034"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/pages | "5026-5034"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/title | "Differential processing of colony-stimulating factor 1 precursors encoded by two human cDNAs."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/title | "Differential processing of colony-stimulating factor 1 precursors encoded by two human cDNAs."xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/3264877 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3264877 |
| http://purl.uniprot.org/citations/3264877 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3264877 |
| http://purl.uniprot.org/citations/3264877 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3264877 |
| http://purl.uniprot.org/citations/3264877 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3264877 |
| http://purl.uniprot.org/uniprot/P09603 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/3264877 |
| http://purl.uniprot.org/uniprot/P09603#attribution-F5C7B8A0F89E975926530FA311D98C41 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/3264877 |