http://purl.uniprot.org/citations/6780981 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6780981 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6780981 | http://www.w3.org/2000/01/rdf-schema#comment | "The mRNA for alcohol dehydrogenase (ADH) in D. melanogaster has been identified by translation in a cell-free system. The in vitro synthesized polypeptide, specifically precipitated by anti-ADH antibody, has identical subunit molecular weight (25,000 daltons) and tryptic peptide profile to the in vivo synthesized ADH. The poly A containing ADH-mRNA has been purified by specific precipitation of ADH-polysomes using anti-ADH antibody and S. aureus. Transformation of E. coli with the dA-tailed ADH-mRNA-complementary DNA hybrid annealed to the dT-tailed pBR322 yielded one plasmid which has been identified as the ADH-cDNA clone. The identification involved hybridization selection of ADH-mRNA and in vitro translation, in situ hybridization to the Adh locus on salivary gland polytene chromosomes and DNA sequencing. This ADH-cDNA plasmid contains 349 bases of the C-terminal protein coding and 180 bases of the 3' untranslated region."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/8.23.5649"xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/8.23.5649"xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Wang N."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Wang N."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Benyajati C."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Benyajati C."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Reddy A."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Reddy A."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Sofer W."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Sofer W."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Weinberg E."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/author | "Weinberg E."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/date | "1980"xsd:gYear |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/date | "1980"xsd:gYear |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/pages | "5649-5667"xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/pages | "5649-5667"xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/title | "Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/title | "Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone."xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/volume | "8"xsd:string |
http://purl.uniprot.org/citations/6780981 | http://purl.uniprot.org/core/volume | "8"xsd:string |