| http://purl.uniprot.org/citations/7188854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7188854 | http://www.w3.org/2000/01/rdf-schema#comment | "Somatomedin-C (SM-C) was purified from Cohn fraction IV of human plasma by a series of steps which included cation-exchange chromatography, gel filtration, isoelectric focusing, and reverse-phase high-pressure liquid chromatography. The peptide isolated contained 10,142 units/mg, as judged by a radioimmunoassay, representing a 780,000-fold purification from native plasma. The isolated peptide was basic (pI 8.1-8.5) and was judged to be no less than 90% pure. The best fit for integral amino acids was obtained with 78 residues. Limited sequence data were obtained on the N terminus and on five fragments obtained by tryptic digestion after blocking the lysine residues. Three of these fragments and the N terminus could be aligned with portions of insulin-like growth factor I (IGF-I). Of the 25 residues so aligned, 22 were identical with IGF-I. In addition, two tryptic fragments were obtained which are not present in the sequence of IGF-I. SM-C and IGF-I produced identical curves of displacement in radioimmunoassay and radioreceptor assays for SM-C and in an insulin radioreceptor assay. The potencies of SM-C and IGF-I in these assays differed significantly from IGF-II, somatomedin-A, and several preparations of multiplication stimulating activity. At a concentration of 1 ng/mL, SM-C stimulated the progression of Balb/c 3T3 cells into DNA synthesis and when injected in vivo restored mitosis in lens epithelium of hypophysectomized frogs."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00545a027"xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Klapper D.G."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Fellows R.E."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Svoboda M.E."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Van Wyk J.J."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Grissom F.E."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/author | "Schlueter R.J."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/date | "1980"xsd:gYear |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/pages | "790-797"xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/title | "Purification of somatomedin-C from human plasma: chemical and biological properties, partial sequence analysis, and relationship to other somatomedins."xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/7188854 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7188854 |
| http://purl.uniprot.org/citations/7188854 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7188854 |
| http://purl.uniprot.org/uniprot/P05019#attribution-AE0EF9C3EFF72440BBE098043668DBFC | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/7188854 |