| http://purl.uniprot.org/citations/7476978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7476978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7476978 | http://www.w3.org/2000/01/rdf-schema#comment | "Serine 118 is definitively identified as a major site of phosphorylation in the human estrogen receptor expressed in COS-1 cells treated with estradiol or phorbol ester. At least 30% of the estrogen receptor appears to be phosphorylated on serine 118 after treatment with estradiol or phorbol ester. Human estrogen receptor was expressed in COS-1 cells and labeled in vivo with [32P]orthophosphate in the presence of estradiol or phorbol ester. Immunopurified receptor was digested with cyanogen bromide. The most heavily labeled peptide (7 kilodaltons) was identified as amino acids 110-174 by microsequencing. Manual Edman degradation released a major portion of the 32P-label in the peptide at serine 118. A mutant with serine 118 replaced by alanine (S118A) had 80% less 32P-label in the 7 kilodalton peptide. Estrogen receptor labeled in vivo with [32P]-orthophosphate in the presence of estradiol or phorbol ester migrates electrophoretically as a doublet. The major difference between the bands is phosphorylation of serine 118 in the upshifted band. The mutant S118A does not show an upshifted band. Labeling of the estrogen receptor with [35S]methionine indicates that > or = 30% of the receptor is upshifted and suggests that > or = 30% of the receptor is phosphorylated on serine 118."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.9.8.7476978"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.9.8.7476978"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Traish A.M."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Traish A.M."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Joel P.B."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Joel P.B."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Lannigan D.A."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/author | "Lannigan D.A."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/pages | "1041-1052"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/pages | "1041-1052"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/title | "Estradiol and phorbol ester cause phosphorylation of serine 118 in the human estrogen receptor."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/title | "Estradiol and phorbol ester cause phosphorylation of serine 118 in the human estrogen receptor."xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/7476978 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7476978 |
| http://purl.uniprot.org/citations/7476978 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7476978 |
| http://purl.uniprot.org/citations/7476978 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7476978 |
| http://purl.uniprot.org/citations/7476978 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7476978 |