| http://purl.uniprot.org/citations/7522053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7522053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7522053 | http://www.w3.org/2000/01/rdf-schema#comment | "Active PAI-1 (plasminogen activator inhibitor 1) is bound to vitronectin in plasma and in the extracellular matrix. In this study we aimed at identifying the PAI-1 binding site in vitronectin, which at present is a matter of dispute. Vitronectin was cleaved with trypsin and the fragments were tested for inhibitory effect on the PAI-1/vitronectin interaction using vitronectin-coated microtiter plates. Intact vitronectin and the tryptic digest of vitronectin both caused a 50% reduction in PAI-1 binding at a concentration of about 2 nmol/I. Gel-filtration on Sephadex G-50 superfine of the tryptic peptides resulted in one main peak of inhibitory activity. The elution volume, Kav, was 0.55 indicating (a) medium-size peptide(s). The peptide was further purified by reverse-phase HPLC. Structural analysis revealed that it constituted the 45 NH2-terminal amino-acid residues in vitronectin. The NH2-terminal vitronectin peptide caused a 50% decrease in PAI-1 binding to the vitronectin-coated microtiter plates at a concentration of about 13 nmol/l. Thus, the peptide is a little less effective in this respect than intact vitronectin. Reduced and S-carboxymethylated peptide had no effect on the interaction. The NH2-terminal vitronectin fragment increased the stability of active PAI-1 by about 60%, which is a little less than with intact vitronectin. The peptide also prevented PAI-1 from oxidation with chloramine T. The half-life was prolonged about 4-fold as compared to about 30-fold with intact vitronectin."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.org/dc/terms/identifier | "doi:10.1016/0167-4838(94)90166-x"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.org/dc/terms/identifier | "doi:10.1016/0167-4838(94)90166-x"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/author | "Wiman B."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/author | "Wiman B."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/author | "Sigurdardottir O."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/author | "Sigurdardottir O."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/pages | "104-110"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/pages | "104-110"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/title | "Identification of a PAI-1 binding site in vitronectin."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/title | "Identification of a PAI-1 binding site in vitronectin."xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/volume | "1208"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://purl.uniprot.org/core/volume | "1208"xsd:string |
| http://purl.uniprot.org/citations/7522053 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7522053 |
| http://purl.uniprot.org/citations/7522053 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7522053 |
| http://purl.uniprot.org/citations/7522053 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7522053 |
| http://purl.uniprot.org/citations/7522053 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7522053 |
| http://purl.uniprot.org/uniprot/P05121 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7522053 |
| http://purl.uniprot.org/uniprot/P04004 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7522053 |